Stimulation of secretion of gastric inhibitory polypeptide and insulin by intraduodenal amino acid perfusion.
Stimulation of secretion of gastric inhibitory polypeptide and insulin by intraduodenal amino acid perfusion.
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DOI：
10.1016/s0016-5085(76)80489-1
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发表时间：
1976-04
期刊：
影响因子：
29.4
通讯作者：
Fred B. Thomas;E. L. Mazzaferri;S. Crockett;Hagop S. Mekhjian;Hans D. Gruemer;Samuel Cataland
中科院分区：
文献类型：
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作者：
Fred B. Thomas;E. L. Mazzaferri;S. Crockett;Hagop S. Mekhjian;Hans D. Gruemer;Samuel Cataland
在 10 名正常受试者中，研究了十二指肠内或静脉内给予 30 gm 混合氨基酸溶液对血清抑胃多肽 (GIP)、α-氨基氮 (AAN)、葡萄糖和胰岛素浓度的影响。最初，对每个受试者进行十二指肠内氨基酸灌注（15 ml/min × 60 min），然后在 1 至 2 周内进行静脉输注。两种给药途径后 60 分钟出现 AAN 浓度峰值，但静脉输注时浓度更高，分别为 145 ± 5.7 μg/ml 和 89 ± 4.4 μg/ml (P< 0.001)。尽管十二指肠内给药后血清AAN水平显着降低，但十二指肠内灌注后胰岛素浓度增量更大，为77.3±8.8μU/ml vs. 43.1±5.6μU/ml(P<0.005)。十二指肠内氨基酸后，总积分胰岛素分泌也更大，5000 vs. 2400 μU-min ml-1（P<0.005）。静脉输注氨基酸后，血清 GIP 浓度仍低于测定检测限。十二指肠内灌注后，15 分钟时平均最大 GIP 增量为 468 pg/ml。所有受试者的 GIP 浓度峰值出现在 15 分钟，并且比胰岛素最大上升时间早 15 至 30 分钟。十二指肠内氨基酸灌注后，总整合 GIP 分泌显着增加，13,000 pg-min ml-1vs。静脉输注没有可测量的反应。在对 12 名受试者进行的单独研究中，十二指肠内灌注 0.45% 盐水、0.9% 盐水或 10% 甘露醇后，血清 GIP 浓度没有发生显着变化。这项研究的结果表明，十二指肠内施用氨基酸会刺激 GIP 的分泌，并表明内源性释放的 GIP 在肠介导的胰岛素释放中可能很重要。
The effect of intraduodenal or intravenous administration of a 30-gm mixed amino acid solution on serum gastric inhibitory polypeptide (GIP), α-amino nitrogen (AAN), glucose, and insulin concentrations was studied in 10 normal subjects. Initially, an intraduodenal amino acid perfusion (15 ml per min × 60 min) was performed in each subject and was followed in 1 to 2 weeks by an intravenous infusion. Peak AAN concentrations occurred at 60 min after both routes of administration, but were greater with intravenous infusion, 145 ± 5.7 μg per ml vs. 89 ± 4.4 μg per ml (P< 0.001). Although serum AAN levels were significantly lower after intraduodenal administration, incremental insulin concentrations were greater after intraduodenal perfusion, 77.3 ± 8.8 μU per ml vs. 43.1 ± 5.6 μU per ml (P< 0.005). Total integrated insulin secretion was also greater after intraduodenal amino acids, 5000 vs. 2400 μU-min ml-1(P< 0.005). With intravenous amino acid infusion, serum GIP concentrations remained below the assay detection limit. After intraduodenal perfusion, a mean maximum GIP increment of 468 pg per ml occurred at 15 min. In all subjects peak GIP concentrations occurred at 15 min and preceded the maximum insulin rise by 15 to 30 min. Total integrated GIP secretion was significantly greater after intraduodenal amino acid perfusion, 13,000 pg-min ml-1vs. no measurable response with intravenous infusion. In separate studies performed in 12 subjects, no significant changes in serum GIP concentrations occurred after intraduodenal perfusion of 0.45% saline, 0.9% saline, or 10% mannitol. The results of this study demonstrate that intraduodenal amino acid administration stimulates the secretion of GIP and suggest that endogenously released GIP may be important in the enteric mediated release of insulin.