MHC-unrestricted lysis of MUC1-expressing cells by human peripheral blood mononuclear cells.
MHC-unrestricted lysis of MUC1-expressing cells by human peripheral blood mononuclear cells.
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人外周血单核细胞对表达 MUC1 的细胞进行 MHC 无限制裂解。
DOI：
10.1080/08820130801967874
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发表时间：
2008
影响因子：
2.8
通讯作者：
Philip,Ramila
中科院分区：
文献类型：
--
作者：
Wright,StephenE;Rewers-Felkins,KathleenA;Quinlin,ImeldaS;Fogler,WilliamE;Phillips,CatherineA;Townsend,Mary;Robinson,William;Philip,Ramila
许多人腺癌可以在体外被靶向细胞毒性T淋巴细胞（CTL）杀死;然而，通常需要主要组织相容性复合体（MHC）限制。MUC 1抗原在许多人类腺癌中是常见的，并且与可变数目的串联重复相关。已经提出，具有这种重复表位的抗原在没有MHC限制的情况下可能易受细胞毒性T淋巴细胞杀伤。因此，在没有MHC限制的情况下，表达MUC 1的恶性细胞可能被靶向细胞毒性T淋巴细胞杀死。在这项研究中，一个人MUC 1表达的小鼠乳腺癌细胞系被用来确定是否需要MHC限制的MUC 1表达腺癌细胞的细胞毒性T淋巴细胞杀伤。具体而言，观察到MUC 1刺激的人单核细胞（M1 SMC）杀死人MUC 1转染的、表达MUC 1的鼠乳腺癌细胞，但不杀死模拟转染的、不表达MUC 1的鼠乳腺癌细胞。此外，该杀伤被MUC 1抗体阻断，表明MUC 1特异性杀伤。总之，MUC 1表达腺癌细胞的细胞毒性T淋巴细胞杀伤可以是MHC不受限制的。
Many human adenocarcinomas can be killedin vitroby targeted cytotoxic T-lymphocytes (CTL); however, major histocompatibility complex (MHC)-restrictions are typically required. The MUC1 antigen is common in many human adenocarcinomas, and is associated with a variable number of tandem repeats. It has been proposed that antigens with such repeated epitopes may be vulnerable to cytotoxic T-lymphocyte killing without MHC-restriction. Therefore, it is possible that MUC1-expressing malignant cells may be killed by targeted cytotoxic T-lymphocyte in the absence of MHC-restriction. In this study, a human MUC1-expressing murine mammary carcinoma cell line was used to determine if cytotoxic T-lymphocyte killing of MUC1-expressing adenocarcinoma cells requires MHC-restriction. Specifically, MUC1-stimulated human mononuclear cells (M1SMC) were observed to kill human MUC1-transfected, MUC1-expressing murine mammary carcinoma cells, but not the mock-transfected, non-MUC1-expressing murine mammary carcinoma cells. Furthermore, the killing was blocked by antibody to MUC1, indicating MUC1-specific killing. In conclusion, cytotoxic T-lymphocyte killing of MUC1-expressing adenocarcinoma cells can be MHC-unrestricted.