Evaluation of an Inexpensive Growth Medium for Direct Detection of Escherichia coli in Temperate and Sub-Tropical Waters.
Evaluation of an Inexpensive Growth Medium for Direct Detection of Escherichia coli in Temperate and Sub-Tropical Waters.
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DOI：
10.1371/journal.pone.0140997
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Pedley S
中科院分区：
文献类型：
--
作者：
Bain RE;Woodall C;Elliott J;Arnold BF;Tung R;Morley R;du Preez M;Bartram JK;Davis AP;Gundry SW;Pedley S
传统的粪便指示菌检测方法的成本和复杂性，包括大肠杆菌。大肠杆菌，阻碍了饮用水质量的广泛监测，特别是在低收入国家和外部控制的实验室环境。在这些环境中，由于缺乏用于检测大肠杆菌的廉价培养基，问题变得更加严重。饮用水中的大肠杆菌。我们开发了一种新的低成本生长培养基，aquatest（AT），并验证了其用于直接检测E。使用IDEXX Quanti-Tray®检测温带和亚热带饮用沃茨中的大肠杆菌。将AT与IDEXX Colilert-18®和EC-MUG或MLSB进行比较，以检测低水平的E。来自温带（n = 140;布里斯托，英国）和亚热带地区（n = 50，比勒陀利亚/茨瓦内，南非）的水样的大肠杆菌。使用验证性测试（分别为n = 418和588）和定量结果的比较来评估性能。对于英国[98.0% vs. 86.9%; p<0.0001]和南非[99.5% vs. 93.2%; p = 0.0030]的水样，AT的灵敏度高于Colilert-18®。特异性没有显著差异，两种培养基的特异性都很高（两种情况下>95%）。定量结果具有可比性，且在预期限度内。AT检测E.温带和亚热带饮用水中的大肠杆菌。本文报道了新培养基的组成，可自由使用。
The cost and complexity of traditional methods for the detection of faecal indicator bacteria, including E. coli, hinder widespread monitoring of drinking water quality, especially in low-income countries and outside controlled laboratory settings. In these settings the problem is exacerbated by the lack of inexpensive media for the detection of E. coli in drinking water. We developed a new low-cost growth medium, aquatest (AT), and validated its use for the direct detection of E. coli in temperate and sub-tropical drinking waters using IDEXX Quanti-Tray®. AT was compared with IDEXX Colilert-18® and either EC-MUG or MLSB for detecting low levels of E. coli from water samples from temperate (n = 140; Bristol, UK) and subtropical regions (n = 50, Pretoria/Tshwane, South Africa). Confirmatory testing (n = 418 and 588, respectively) and the comparison of quantitative results were used to assess performance. Sensitivity of AT was higher than Colilert-18® for water samples in the UK [98.0% vs. 86.9%; p<0.0001] and South Africa [99.5% vs. 93.2%; p = 0.0030]. There was no significant difference in specificity, which was high for both media (>95% in both settings). Quantitative results were comparable and within expected limits. AT is reliable and accurate for the detection of E. coli in temperate and subtropical drinking water. The composition of the new medium is reported herein and can be used freely.