Intimal lining layer macrophages but not synovial sublining macrophages display an IL-10 polarized-like phenotype in chronic synovitis
Intimal lining layer macrophages but not synovial sublining macrophages display an IL-10 polarized-like phenotype in chronic synovitis
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DOI：
10.1186/ar3796
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发表时间：
2012-01-01
影响因子：
4.9
通讯作者：
Baeten, Dominique L. P.
中科院分区：
文献类型：
--
作者：
Ambarus, Carmen A.;Noordenbos, Troy;Baeten, Dominique L. P.
简介：滑膜组织巨噬细胞在慢性炎症性关节炎中起关键作用，但不同巨噬细胞亚群在此过程中的作用仍不清楚。主要的体外极化巨噬细胞亚群是经典（M1）和交替（M2）活化的巨噬细胞，后者包括白介素（IL）-4和IL-10极化细胞。方法：采用免疫组化和流式细胞术分别检测脊柱关节炎（SpA）和类风湿关节炎（RA）患者滑膜巨噬细胞、外周血单核细胞和体外极化的单核细胞源性巨噬细胞的极化标志物的表达。结果：与RA内膜衬里层相比，SpA内膜衬里层中IL-10极化标志物CD 163的表达明显增加，而M1和M2标志物在两种疾病间无明显差异。此外，在单核细胞和体外极化单核细胞衍生的巨噬细胞之间没有显着的表型差异，观察到SpA，RA，和健康对照，表明差异CD 163的表达并不反映在SpA的优先M2极化。内膜衬里层巨噬细胞的更详细的分析显示，在SpA和RA中，IL-10极化标志物CD 163和分化簇32（CD 32）强烈共表达，但没有任何其他标志物。相比之下，滑膜下层巨噬细胞有一个更异质性的表型，与大多数细胞共表达M1和M2 markets.Conclusions：内膜衬里层，但不是滑膜下层巨噬细胞显示IL-10极化样表型，增加CD 163的表达在SpA与RA滑膜炎。极化巨噬细胞亚群分布的这些差异可能有助于慢性滑膜炎的结果。
Introduction: Synovial tissue macrophages play a key role in chronic inflammatory arthritis, but the contribution of different macrophage subsets in this process remains largely unknown. The main in vitro polarized macrophage subsets are classically (M1) and alternatively (M2) activated macrophages, the latter comprising interleukin (IL)-4 and IL-10 polarized cells. Here, we aimed to evaluate the polarization status of synovial macrophages in spondyloarthritis (SpA) and rheumatoid arthritis (RA).Methods: Expression of polarization markers on synovial macrophages, peripheral blood monocytes, and in vitro polarized monocyte-derived macrophages from SpA versus RA patients was assessed by immunohistochemistry and flow cytometry, respectively. The polarization status of the intimal lining layer and the synovial sublining macrophages was assessed by double immunofluorescence staining.Results: The expression of the IL-10 polarization marker cluster of differentiation 163 (CD163) was increased in SpA compared with RA intimal lining layer, but no differences were found in other M1 and M2 markers between the diseases. Furthermore, no significant phenotypic differences in monocytes and in vitro polarized monocyte-derived macrophages were seen between SpA, RA, and healthy controls, indicating that the differential CD163 expression does not reflect a preferential M2 polarization in SpA. More detailed analysis of intimal lining layer macrophages revealed a strong co-expression of the IL-10 polarization markers CD163 and cluster of differentiation 32 (CD32) but not any of the other markers in both SpA and RA. In contrast, synovial sublining macrophages had a more heterogeneous phenotype, with a majority of cells co-expressing M1 and M2 markers.Conclusions: The intimal lining layer but not synovial sublining macrophages display an IL-10 polarized-like phenotype, with increased CD163 expression in SpA versus RA synovitis. These differences in the distribution of the polarized macrophage subset may contribute to the outcome of chronic synovitis.