Application of PCR for detection of mycoplasma DNA and pestivirus RNA in human live viral vaccines.
Application of PCR for detection of mycoplasma DNA and pestivirus RNA in human live viral vaccines.
复制标题
应用PCR检测人活病毒疫苗中支原体DNA和瘟病毒RNA。
DOI：
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发表时间：
1996
期刊：
影响因子：
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通讯作者：
F. Chino
中科院分区：
文献类型：
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作者：
T. Sasaki;R. Harasawa;M. Shintani;H. Fujiwara;Y. Sasaki;A. Horino;T. Kenri;K. Asada;I. Kato;F. Chino
应用聚合酶链式反应技术对日本6家厂家生产的43批疫苗(麻疹、腮腺炎、风疹、口服脊髓灰质炎)进行了支原体DNA和鼠疫病毒RNA的检测。虽然在所测试的任何疫苗中都没有检测到支原体DNA，但在12批(28%)中检测到了鼠疫病毒RNA。4种病毒疫苗之间的污染发生率在20%到37%之间，6家厂家之间的污染发生率在0%到56%之间。
PCR techniques were applied for the detection of mycoplasma DNA and pestivirus RNA to 43 lots of live viral vaccines (measles, mumps, rubella, and oral poliomyelitis) produced by six manufacturers in Japan. Although mycoplasma DNA was not detected in any of the vaccines tested, pestivirus RNA was detected in 12 lots (28%). The incidence of contamination among the four viral vaccines was in the range of 20 to 37%, and the incidence among the six manufacturers varied from 0 to 56%.