Simultaneous UPLC MS/MS analysis of endogenous jasmonic acid, salicylic acid, and their related compounds
Simultaneous UPLC MS/MS analysis of endogenous jasmonic acid, salicylic acid, and their related compounds
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DOI：
10.1007/s10725-008-9347-7
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发表时间：
2009-04-01
影响因子：
4.2
通讯作者：
Nabeta, Kensuke
中科院分区：
文献类型：
--
作者：
Matsuura, Hideyuki;Aoi, Arata;Nabeta, Kensuke
茉莉酸（JA）和水杨酸（SA）是植物激素，参与植物的生长和发育。最近的研究表明，JA和SA信号通路之间存在复杂的相互作用，以应对病原攻击和生物胁迫。据我们所知，还没有同时分析JA、SA及其相关化合物的方法。特别是糖苷类化合物被认为是贮存或失活的化合物，但在阐明糖苷配基的含量时应考虑它们的贡献。由于异分支酸途径的存在，它也是有价值的测定内源量的苯丙氨酸，肉桂酸，苯甲酸的生物合成的中间体SA。我们建立了以氘标记化合物为内标的方法。这是第一个报告的同时分析内源性JA，SA，及其相关化合物。测定烟草植株中积累的内源JA、SA及其相关化合物证明了新开发的方法的实用性。结果表明，在TMV感染和非生物胁迫条件下，茉莉酸、水杨酸及其相关化合物的积累均受到诱导。
Jasmonic acid (JA) and salicylic acid (SA) are plant hormones involved in plant growth and development. Recent studies demonstrated that presence of a complex interplay between JA and SA signaling pathways to response to pathogenesis attack and biotic stresses. To our best knowledge, no method has existed for simultaneous analyses of JA, SA, and their related compounds. Especially, the glucosides are thought to be the storages or the inactivated compounds, but their contribution should be considered for elucidating the amount of the aglycons. It is also valuable for measuring the endogenous amount of phenylalanine, cinnamic acid, and benzoic acid that are the biosynthetic intermediates of SA due to the existence of isochorismate pathway to synthesize SA. We established this method using deuterium labeled compounds as internal standards. This is the first report of simultaneous analysis of endogenous JA, SA, and their related compounds. Measuring the endogenous JA, SA, and their related compounds that had been accumulated in tobacco plants proved the practicality of the newly developed method. It was demonstrated that accumulation of JA, SA and their related compounds were induced in both case of TMV infection and abiotic stresses.