A truncated isoform of Ca2+/calmodulin-dependent protein kinase II expressed in human islets of Langerhans may result from trans-splicing
A truncated isoform of Ca2+/calmodulin-dependent protein kinase II expressed in human islets of Langerhans may result from trans-splicing
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DOI：
10.1016/s0014-5793(97)00555-3
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发表时间：
1997-06-16
期刊：
影响因子：
3.5
通讯作者：
Ashcroft, SJH
中科院分区：
文献类型：
--
作者：
Breen, MA;Ashcroft, SJH
钙/钙调蛋白依赖性蛋白激酶II利用cDNA末端快速扩增技术，从人胰岛RNA中扩增出CaM激酶Ⅱ γ基因的3'末端，检测到一种新的cDNA，其由来自人CaM激酶II γ基因的5'序列连接到人信号识别颗粒的3'末端组成72（SRP 72）基因。我们预测，这种mRNA的物种将编码的截断形式的钙调蛋白激酶II，指定的γ（SRP），包括整个催化和调节结构域的蛋白质和预测的分子量为37 kDa。我们将人类SRP 72基因定位于18号染色体，并且由于CaM激酶II γ基因先前定位于人类染色体10 q22，我们认为这种新的cDNA可能是由反式剪接引起的。(C)1997年欧洲生物化学学会联合会。
Calcium/calmodulin-dependent protein kinase II (CaM kinase II) has been proposed to play a key role in glucose stimulated insulin secretion, Using the rapid amplification of cDNA ends technique we amplified the 3' end of the CaM kinase II gamma gene from human islet RNA, A novel cDNA was detected composed of 5' sequence from the human CaM kinase II gamma gene joined to the 3' end of the human signal recognition particle 72 (SRP72) gene. We predict that this mRNA species will code for a truncated form of CaM kinase II, designated gamma(SRP), comprising the entire catalytic and regulatory domains of the protein and with a predicted molecular weight of 37 kDa. We mapped the human SRP72 gene to chromosome 18 and, as the CaM kinase II gamma gene was previously mapped to human chromosome 10q22, we suggest this novel cDNA may have resulted from transsplicing. (C) 1997 Federation of European Biochemical Societies.