The skeletal proteome of the brittle star Ophiothrix spiculata identifies C-type lectins and other proteins conserved in echinoderm skeleton formation
The skeletal proteome of the brittle star Ophiothrix spiculata identifies C-type lectins and other proteins conserved in echinoderm skeleton formation
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DOI：
10.3934/molsci.2016.3.357
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发表时间：
2016-01-01
影响因子：
1.4
通讯作者：
Livingston, Brian T.
中科院分区：
文献类型：
--
作者：
Flores, Rachel L.;Gonzales, Kayla;Livingston, Brian T.
确定参与生物矿化和骨骼形成的蛋白质的身份和功能作用对我们理解这一过程至关重要。蛋白质组学已经允许快速表征矿化组织中的蛋白质，但是检测到的大量蛋白质使得难以分配每个蛋白质的相对重要性。我们已经采取了比较的方法，检查不同种类的棘皮动物的骨骼蛋白质组，以确定保守的蛋白质，可能是重要的。我们先前的研究比较了脆星星Ophiocoma wendtii的骨骼蛋白质组和已发表的海胆Strongylocentrotus purpuratus的蛋白质组，揭示了一些蛋白质的保守性，但表明在海胆骨骼蛋白质组中丰富的C型凝集素结构域的骨针基质蛋白在脆星星中不保守。在这里，我们研究了骨骼蛋白质组的不同种类的脆星星，蛇针。我们从O.并进行LC/MS/MS以鉴定存在的肽。与转录组和基因组数据库的比较揭示了O.针形藻蛋白质组尽管已经分开了几百万年，但这两颗脆性恒星的骨骼中有非常相似的蛋白质。包括纤维蛋白原C样凝集素和几个C型凝集素蛋白，我们详细描述。在S. Purpuatus骨架蛋白和这些骨架蛋白中的重复区域在O.刺状的。
Determining the identity and functional role of proteins involved in biomineralization and the formation of skeletons is critical to our understanding of the process. Proteomics has allowed rapid characterization of the proteins occluded within mineralized tissue, but the large numbers of proteins detected makes it difficult to assign the relative importance of each protein. We have taken a comparative approach, examining the skeletal proteome of different species of echinoderms in order to identify the proteins that are conserved and likely to be important. Our previous study comparing the skeletal proteome of the brittle star Ophiocoma wendtii to the published proteomes of the sea urchin Strongylocentrotus purpuratus revealed some conservation of proteins, but indicated that the C-type lectin domain-containing spicule matrix proteins abundant in the sea urchin skeletal proteome were not conserved in the brittle star. Here we examine the skeletal proteome of a different species of brittle star, Ophiothrix spiculata. We have isolated the proteins from the skeleton of O. spiculata and performed LC/MS/MS to identify peptides present. Comparison to transcriptome and genome databases revealed the proteins present in the O. spiculata proteome. Despite being diverged for several million years, the two brittle stars have very similar proteins in their skeletons. Included is a fibrinogen C-like lectin and several C-type lectins proteins, which we describe in detail. The unusual number of C-type lectins found in the S. purpuatus skeleton and the repetitive regions seen in those spicule matrix proteins are not present in O. spiculata.