Application of the high mass accuracy capabilities of FT-ICR-MS and Q-ToF-MS to the characterisation of arsenic compounds in complex biological matrices
Application of the high mass accuracy capabilities of FT-ICR-MS and Q-ToF-MS to the characterisation of arsenic compounds in complex biological matrices
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应用 FT-ICR-MS 和 Q-ToF-MS 的高质量准确度功能来表征复杂生物基质中的砷化合物
DOI：
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
J. Thomas
中科院分区：
文献类型：
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作者：
R. Pickford;M. Miguens‐Rodriguez;S. Afzaal;Paul Speir;S. Pergantis;J. Thomas
本通讯详细介绍了我们在电喷雾质谱仪应用于砷形态分析领域的最新进展。详细的实验证明，在含有至少50种不同化合物的生物提取物的电喷雾质谱图中，可以实现对单个含砷化合物的识别。现代质谱仪可获得的卓越质量精度和分辨率使这一过程成为可能。本工作首次将傅里叶变换离子回旋共振质谱仪(FT-ICR)应用于有机砷化合物的分析。利用FT-ICR优良的质量准确度来指认元素组成，从而识别从含砷化合物中产生的离子。这延伸到串联质谱学数据，其中产物离子可以被指定为含砷或不含砷。由于FT-ICR仪器价格昂贵
而且大多数砷化学家并不容易获得这种为FT-ICR使用而开发的方法，还使用混合四极飞行时间(Q-ToF)仪器进行了评估。结果表明，使用这些仪器获得的低百万分率(Ppm)质量精度，尽管不如FT-ICR技术所能获得的那样高，但仍然能够表征粗分海带提取物中的含砷化合物。这些结果为快速识别和随后鉴定粗提取物中未知的砷物种提供了新的视角，而不需要广泛的提纯或先前表征的标准。
This communication details our recent developments in the application of electrospray mass spectrometry to the field of arsenic speciation. The experiments detailed demonstrate that recognition of individual arsenic-containing compounds can be achieved in electrospray mass spectra of biological extracts containing at least fifty different compounds. The excellent mass accuracy and resolution obtainable with modern mass spectrometers makes this process possible. This work demonstrates the first application, as far as the authors are aware, of Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR) to the analysis of organoarsenic compounds. The excellent mass accuracy of FT-ICR is exploited to assign elemental compositions and thus identify ions generated from arsenic-containing compounds. This extends to the tandem mass spectral data, where product ions could be assigned as arsenic-containing or non-arsenic-containing. As FT-ICR instrumentation is expensive
and not readily available to most arsenic-chemists the approach developed for use with FT-ICR was also evaluated using a hybrid quadrupole–time-of-flight (Q-ToF) instrument. It is shown that the low parts per million (ppm) mass accuracies obtained using these instruments, although not as great as those obtainable with FT-ICR technology, still enable the characterisation of arsenic-containing compounds in a crudely fractionated kelp extract. These results offer new perspectives for the rapid recognition, and subsequent identification, of unknown arsenic species in crude extracts, without the need for extensive purification or previously characterised standards.
影响因子：
7.4
作者：
Enke, CG
通讯作者：
Enke, CG