Quercetin treatment against ischemia/reperfusion injury in rat corpus cavernosum tissue: a role on apoptosis and oxidative stress
Quercetin treatment against ischemia/reperfusion injury in rat corpus cavernosum tissue: a role on apoptosis and oxidative stress
复制标题
DOI：
10.3109/10715762.2013.814912
复制
发表时间：
2013-09-01
影响因子：
3.3
通讯作者：
Sener, G.
中科院分区：
文献类型：
--
作者：
Cevik, O.;Cadirci, S.;Sener, G.
活性氧代谢物在缺血/再灌注（I/R）诱导的组织损伤中起重要作用。本研究旨在探讨槲皮素对大鼠海绵体组织I/R损伤的保护作用。为了诱导I/R损伤，夹腹主动脉30min，再灌注60min。I/R组和假手术对照组在缺血前和再灌注后分别给予槲皮素（20 mg/kg）或代药。斩首后，将海绵体组织取出，放入器官浴中或保存以评估生化参数。采用荧光素化学发光（CL）、一氧化氮（NO）、丙二醛（MDA）和谷胱甘肽（GSH）水平、超氧化物歧化酶（SOD）和髓过氧化物酶（MPO）活性和caspase-3蛋白水平检测氧化损伤。在I/R组中，与对照组相比，对苯肾上腺素的收缩反应和对氨基酚的松弛反应明显受损，而在I/R组中槲皮素治疗逆转了这两种反应。另一方面，槲皮素处理显著逆转了I/R组海海绵组织中lucigenin CL、NO、MDA水平升高，MPO和caspase-3活性升高，GSH水平和SOD活性降低。此外，在槲皮素处理的I/R组中，I/R海绵体组织的形态扭曲、内皮细胞破裂和细胞质空泡化不再持续。因此，我们的研究结果表明槲皮素治疗可能通过其抗炎、抗凋亡和抗氧化作用在控制I/ r诱导的组织损伤方面具有一定的益处。
Reactive oxygen metabolites play an important role in the ischemia/reperfusion (I/R)-induced tissue injury. This study was designed to investigate the possible protective effects of quercetin against I/R injury of the rat corpus cavernosum tissue. To induce I/R injury, abdominal aorta was clamped for 30 min and reperfused for 60 min. Quercetin (20 mg/kg) or vehicle was given before ischemia and just after reperfusion in the I/R group and in the sham-operated control group in which clamping was not performed. After decapitation, corpus cavernosum tissues were removed and either placed in organ baths or stored for evaluating biochemical parameters. Oxidative injury was examined by measuring lucigenin chemiluminescence (CL), nitric oxide (NO), malondialdehyde (MDA) and glutathione (GSH) levels, superoxide dismutase (SOD) and myeloperoxidase (MPO) activities and caspase-3 protein levels. In the I/R group, contractile responses to phenylephrine and relaxation responses to carbachol were impaired significantly compared with those in the control groups, while quercetin treatment in I/R group reversed both of the responses. On the other hand, increase in lucigenin CL, NO, MDA levels and MPO and caspase-3 activities and decrease in GSH levels and SOD activity in the cavernosal tissues of the I/R group were also significantly reversed by quercetin treatment. Furthermore, observed distorted morphology with ruptured endothelial cells and vacuolization in the cytoplasm of cavernosal tissues of I/R no longer persisted in the quercetin-treated I/R group. Thus, our results suggested that treatment with quercetin may have some benefits in controlling I/R-induced tissue injury through its anti-inflammatory, anti-apoptotic, and antioxidant effects.