HOMOLOGOUS RECOMBINATION WITHIN SUBTELOMERIC REPEAT SEQUENCES GENERATES CHROMOSOME SIZE POLYMORPHISMS IN P-FALCIPARUM
HOMOLOGOUS RECOMBINATION WITHIN SUBTELOMERIC REPEAT SEQUENCES GENERATES CHROMOSOME SIZE POLYMORPHISMS IN P-FALCIPARUM
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DOI：
10.1016/0092-8674(88)90097-9
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发表时间：
1988-06-03
期刊：
影响因子：
64.5
通讯作者：
KEMP, DJ
中科院分区：
文献类型：
--
作者：
CORCORAN, LM;THOMPSON, JK;KEMP, DJ
我们目前的限制性酶切图谱的染色体1和2的6个克隆系的恶性疟原虫。这些标记描绘了八个遗传标记的位置，包括五种抗原的基因。在来自不同地区的寄生虫中，染色体结构在中部区域是保守的，但在端粒附近的长度和序列上都是多态的。端粒和邻近序列在大多数恶性疟原虫染色体的末端包含保守结构。然而，亚端粒区是多态性的，并且与重复元件（rep20）的位置一致。rep20的缺失产生在1号或2号染色体的一端或两端缺乏rep20的恶性疟原虫克隆，并且较大的缺失也去除了端粒近端基因。因此，染色体长度多态性在很大程度上可以解释为这些重复序列块内的重组，这一机制在恶性疟原虫重复抗原基因多样性的产生中也很重要。
We present restriction maps for chromosomes 1 and 2 of six cloned lines of P. falciparum. These delineate the locations of eight genetic markers, including genes for five antigens. In parasites from diverse areas, chromosome structure is conserved in central regions but is polymorphic both in length and sequence near the telomeres. The telomeres and adjacent sequences comprise a conserved structure at the ends of most P. falciparum chromosomes. However, the subtelomeric zones are polymorphic and coincide with the locations of a repetitive element (rep20). Deletions of rep20 generate clones of P. falciparum that lack rep20 on one or both ends of chromosomes 1 or 2, and larger deletions remove telomere-proximal genes as well. The chromosome length polymorphisms can therefore be largely explained by recombination within these blocks of repeats, a mechanism that is also important in the generation of diversity in genes for repetitive antigens of P. falciparum.