SPATIAL AND TEMPORAL EXPRESSION PATTERN OF N-CADHERIN CELL-ADHESION MOLECULES CORRELATED WITH MORPHOGENETIC PROCESSES OF CHICKEN EMBRYOS
SPATIAL AND TEMPORAL EXPRESSION PATTERN OF N-CADHERIN CELL-ADHESION MOLECULES CORRELATED WITH MORPHOGENETIC PROCESSES OF CHICKEN EMBRYOS
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DOI：
10.1016/0012-1606(87)90119-9
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发表时间：
1987-03-01
影响因子：
2.7
通讯作者：
TAKEICHI, M
中科院分区：
文献类型：
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作者：
HATTA, K;TAKAGI, S;TAKEICHI, M
n -钙粘蛋白是一种Ca2+依赖性细胞-细胞黏附分子，在小鼠和鸡脑细胞中均有发现。本研究采用免疫组织化学方法测定了n -钙粘蛋白在鸡胚不同阶段的表达规律。n -钙粘蛋白在所有三个原胚层的细胞中均有表达。它的表达在许多组织中是短暂的，但在其他组织中是永久性的。瞬时表达见于肾小管、骨骼肌、间质组织、内胚层器官和表皮，而永久性表达见于神经系统、晶状体和心肌细胞。n -钙粘蛋白的出现或消失通常与细胞的重排、分离或结合等形态发生事件有关。将N-cadherin的表达模式与其他工作者测定的L-CAM和N-CAM的表达模式进行比较，提示多种粘附分子的表达存在一定的调控机制。N-cadherin的表达模式与L-CAM的表达模式基本互补；即N-cadherin出现，L-CAM消失，反之亦然。我们还发现N-cadherin在与L-CAM相同的局部区域表达。N-cadherin的分布与N-CAM相似，但有一些例外。因此，n -钙粘蛋白等细胞-细胞粘附分子的表达似乎受到精确的时空控制，并与发育过程中的多种形态发生事件有关。
N-cadherin is a Ca2+-dependent cell-cell adhesion molecule, which was identified in brain cells of mouse and chicken. In the present study, we have determined the pattern of expression of N-cadherin in chicken embryos at various stages by means of immunohistochemistry. N-cadherin was expressed in cells derived from all three primary germ layers. Its expression was transient in many tissues but permanent in others. The transient expression occurred in nephric tubules, skeletal muscles, mesenchymal tissues, endodermal organs, and epidermis, while the permanent expression occurred in nervous systems, lens, and myocardiac cells. Appearance or disappearance of N-cadherin could be generally correlated with morphogenetic events, such as rearrangement, segregation, or association of cells. Comparison of the expression pattern of N-cadherin with that of L-CAM and N-CAM determined by other workers suggests that there is some mechanism controlling expression of multiple classes of adhesion molecules. The pattern of expression of N-cadherin was generally complementary to that of L-CAM; that is, if N-cadherin appeared, L-CAM disappeared or vice versa. We also found cases in which N-cadherin was expressed in the same local regions as L-CAM. The distribution of N-cadherin was similar to that of N-CAM with some exceptions. Thus, N-cadherin and other cell-cell adhesion molecules seem to be expressed under a precise spatial and temporal control so as to be associated with a variety of morphogenetic events during development.