Surface lmmunoproteomics Reveals Potential Biomarkers in Alicyclobacillus acidoterrestris
Surface lmmunoproteomics Reveals Potential Biomarkers in Alicyclobacillus acidoterrestris
复制标题
表面免疫蛋白质组学揭示了酸土脂环酸芽孢杆菌的潜在生物标志物
DOI：
10.3389/fmicb.2018.03032
复制
发表时间：
2018
影响因子：
5.2
通讯作者：
Yuan Yahong
中科院分区：
文献类型：
--
作者：
Shi Yiheng;Yue Tianli;Zhang Yipei;Wei Jianping;Yuan Yahong
酸土脂环酸芽孢杆菌是一种主要的腐败细菌，可导致全球果汁行业的经济损失。目前的检测方法是耗时的，并表现出降低的特异性和灵敏度。在这项研究中，利用免疫蛋白质组学方法来鉴定A的特异性生物标志物。为开发新的检测方法。提取细胞表面相关蛋白，并通过2-D（二维）凝胶电泳进行分离。免疫印迹分析检测免疫原性蛋白，使用抗A。酸土生菌22个蛋白质点表现出免疫原性切除和相关的点中的18个被成功地确定了基质辅助激光解吸/电离飞行时间串联质谱（MALDI-TOF/TOF MS）。这些蛋白质参与能量和碳水化合物代谢、跨膜转运、氧化应激反应、多肽生物合成和分子结合活性。这是第一份详细鉴定A.酸土。所鉴定的免疫原性蛋白可以作为开发新的检测方法的潜在靶点。
Alicyclobacillus acidoterrestris is a major putrefying bacterium that can cause pecuniary losses in the global juice industry. Current detection approaches are time-consuming and exhibit reduced specificity and sensitivity. In this study, an immunoproteomic approach was utilized to identify specific biomarkers from A. acidoterrestris for the development of new detection methods. Cell surface-associated proteins were extracted and separated by 2-D (two-dimensional) gel electrophoresis. Immunogenic proteins were detected by Western blot analysis using antisera against A. acidoterrestris. Twenty-two protein spots exhibiting immunogenicity were excised and eighteen of the associated spots were successfully identified by matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI-TOF/TOF MS). These proteins were observed to be involved in energy and carbohydrate metabolism, transmembrane transport, response to oxidative stress, polypeptide biosynthesis, and molecule binding activity. This is the first report detailing the identification of cell surface-associated antigens of A. acidoterrestris. The identified immunogenic proteins could serve as potential targets for the development of novel detection methods.