FAILURE OF PRETREATMENT WITH GLUCOCORTICOIDS TO MODIFY PHAGOCYTIC AND BACTERICIDAL CAPACITY OF HUMAN LEUKOCYTES FOR ENCAPSULATED TYPE I PNEUMOCOCCUS
FAILURE OF PRETREATMENT WITH GLUCOCORTICOIDS TO MODIFY PHAGOCYTIC AND BACTERICIDAL CAPACITY OF HUMAN LEUKOCYTES FOR ENCAPSULATED TYPE I PNEUMOCOCCUS
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DOI：
10.1128/jb.89.5.1256-1261.1965
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发表时间：
1965-01-01
影响因子：
3.2
通讯作者：
ADCOCK, MH
中科院分区：
文献类型：
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作者：
ALLISON, F;ADCOCK, MH
在含有完全毒性的I型肺炎球菌的体外系统内，评价了给予糖皮质激素4天对从健康人静脉血中回收的白细胞的杀菌和吞噬功能的影响。通过在合适的表面上散布的粒细胞摄入肺炎球菌，然后通过制备的薄载玻片培养物，允许精确定量细胞吞噬功能。结果发现，接受短期糖皮质激素治疗的健康供体的粒细胞摄入并杀死肺炎球菌，治疗前获得的细胞也是如此。人白细胞与泼尼松龙治疗后获得的功能在高离子强度的悬浮介质中，以及没有细胞治疗前收获，虽然两组的效率显着低于在自体血清中操作时。
The influence of gluco-corticoid administration for 4 days upon the bactericidal and phagocytic functions of leukocytes recovered from venous blood of healthy human beings was evaluated within an in vitro system that contained fully virulent, type I pneumococcus. Ingestion of pneumococcus by granulocytes spread upon a suitable surface, followed by thin slide cultures of the preparation, permitted precise quantitation of cellular phagocytic functions. It was found that granulocytes from healthy donors subjected to short-term glucocorticoid treatment ingested and killed pneumococcus as well as did cells obtained before therapy. Human leukocytes obtained after treatment with prednisolone functioned in a suspending medium of high ionic strength as well as did cells harvested before treatment, although the efficiency of both groups was significantly less than when manipulated in autologous serum.