Long-term maintenance of brain tumor stem cell properties under at non-adherent and adherent culture conditions
Long-term maintenance of brain tumor stem cell properties under at non-adherent and adherent culture conditions
复制标题
DOI：
10.1016/j.bbrc.2007.07.037
复制
发表时间：
2007-09-28
影响因子：
3.1
通讯作者：
Iwama, Toru
中科院分区：
文献类型：
--
作者：
Inagaki, Akihito;Soeda, Akio;Iwama, Toru
越来越多的证据表明恶性脑肿瘤中存在癌干细胞样祖细胞。这种祖细胞亚群，即所谓的“癌症干细胞（CSC）”，可能在脑肿瘤的发生、生长和复发中起关键作用。在这里，我们描述了建立一个永久性的脑肿瘤干细胞系，能够形成新的球体后，在贴壁单层条件下培养，并概括了原始肿瘤移植到免疫缺陷小鼠的属性。重新形成的球体保留了它们的干细胞特性，并且即使在长期培养（超过2年）后，从这些球体中分离的单个CSC也形成球体/肿瘤。这些数据表明，即使在非贴壁/贴壁培养条件下连续传代后，一小群CSC仍保持其干细胞特性。评价潜在的代谢事件和评估这些活细胞系的生物学特征将产生有用的知识，在原位行为的脑肿瘤。(c)2007爱思唯尔公司All rights reserved.
There is increasing evidence for the presence of cancer stem-like progenitors in malignant brain tumors. This subpopulation of progenitor cells, the so-called "cancer stem cells (CSCs)", may play a pivotal role in brain tumor initiation, growth, and recurrence. Here we describe the establishment of one permanent brain tumor stem cell line that able to form new spheres after culture under adherent monolayer conditions and to recapitulate the properties of the original tumor upon transplantation into immunodeficient mice. Re-formed spheres retained their stem cell properties and isolated single CSCs from these spheres formed spheres/tumors even after long-term cultures (over 2 years). These data suggested that a small population of CSCs preserved its stem cell properties even after serial passages under non-adherent/adherent culture conditions. Evaluation of underlying metabolic events and assessment of the biological features of these viable cell lines will yield useful knowledge on the in situ behavior of brain tumors. (c) 2007 Elsevier Inc. All rights reserved.