Combined BMP-2 and FGP-4, but neither factor alone, induces cardiogenesis in non-precardiac embryonic mesoderm
Combined BMP-2 and FGP-4, but neither factor alone, induces cardiogenesis in non-precardiac embryonic mesoderm
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DOI：
10.1006/dbio.1996.0211
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发表时间：
1996-08-25
影响因子：
2.7
通讯作者：
Zhu, XL
中科院分区：
文献类型：
--
作者：
Lough, J;Barron, M;Zhu, XL
该实验室先前的工作表明，心脏形成区域的内胚层细胞鸡胚的HFR内胚层诱导心脏前中胚层细胞的终末心脏分化，免疫染色模式表明HFR内胚层细胞表达果蝇十肢麻痹（dpp）样抗原，提示简并聚合酶链反应（PCR）筛选转化生长因子β（TGF-β）dpp亚组的cDNA。（TGF-β）家族。在50个已测序的PCR产物克隆中，半数以上与骨形态发生蛋白-2（BMP-2）同源。未检测到其它TGF-β cDNA，表明BMP-2是HFR内胚层细胞合成的主要dpp亚群蛋白。然而，BMP-2蛋白并没有促进培养的心前或非心前中胚层细胞的存活。尽管FGF-4支持心前中胚层中的心脏发生，但它不诱导非心前中胚层中的心脏发生。保持外植体的活力。与这些生长因子的单独作用相反，用组合的BMP-2和FGF-4处理非心前中胚层在大多数外植体中诱导心脏发生，如通过形成表达肌节cu-actin的节律性收缩的多细胞囊泡所揭示的。这些发现表明BMP-2和FGF-4具有各自的分化和增殖活性，其组合将细胞指定为心脏谱系。(C)出版社：Academic Press，Inc.
Previous work in this laboratory has shown that endoderm cells in the heart forming region (HFR endoderm) of the chicken embryo induce terminal cardiac differentiation in explanted precardiac mesoderm cells, Immunostaining patterns Indicating that HFR endoderm cells express Drosophila decapentaplegic (dpp)-like antigens prompted a degenerate polymerase chain reaction (PCR) screen to identify cDNAs in the dpp subgroup of the transforming growth factor-beta (TGF-beta) family. Among 50 clones of PCR products that have been sequenced, over half have identity with bone morphogenetic protein-2 (BMP-2). No other TGF-beta cDNAs have been detected, suggesting that BMP-2 is the major dpp subgroup protein synthesized by HFR endoderm cells. However, BMP-2 protein did not promote survival of either precardiac or non-precardiac mesoderm cells in culture. Whereas FGF-4 supports cardiogenesis in precardiac mesoderm, it did not induce cardiogenesis in non-precardiac mesoderm, although. explant viability was maintained. In contrast to the isolated effects of these growth factors, treatment of non-precardiac mesoderm with combined BMP-2 and FGF-4 induced cardiogenesis in the majority of explants, as revealed by the formation of a rhythmically contractile multicellular vesicle that expresses sarcomeric cu-actin. These findings suggest that BMP-2 and FGF-4 possess respective differentiative and proliferative activities, the combination of which specifies cells to the cardiac lineage. (C) 1996 Academic Press, Inc.