Presence of anti-Lepp12 antibody: a marker for diagnostic and prognostic evaluation of visceral leishmaniasis
Presence of anti-Lepp12 antibody: a marker for diagnostic and prognostic evaluation of visceral leishmaniasis
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DOI：
10.1016/j.trstmh.2007.11.003
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发表时间：
2008-02-01
影响因子：
2.2
通讯作者：
Salotra, Poonam
中科院分区：
文献类型：
--
作者：
Kumar, Dhiraj;Srividya, Gurumurthy;Salotra, Poonam
从婴儿利什曼原虫中克隆的重组Lepp 12（rLepp 12）抗原在利什曼原虫中的诊断潜力进行了评估。Donovani感染的蛋白质印迹法。92份血清样本，包括30例活动性黑热病（KA）患者、17例治疗后KA患者（KA-PT）、20例黑热病后皮肤利什曼病（PKDL）患者和25例对照，分析了rLepp 12、rK 39和DAT阳性。在治疗前阶段采集的所有KA样本均为Lepp 12抗体阳性。其中17例进行了治疗后评价（KA-PT），发现其中10例为阴性。这10例阴性病例中有9例对应于脾退化的临床治愈患者。7例治疗后病例为rLepp 12阳性;所有这些病例均对应于被认为临床治愈但继续存在脾脏肿大（>= 5 cm）的患者。用rLepp 12抗原进行的免疫印迹试验发现大多数PKDL患者（18/20）为阴性。基于rLepp 12的蛋白质印迹诊断100%的患者患有内脏疾病，而没有发现对照病例对rLepp 12有反应。rLepp 12蛋白为KA的高敏感性和特异性诊断提供了有用的试剂。此外，rLepp 12似乎有可能作为感染的预后标志物。(c)2007年皇家热带医学和卫生学会。由爱思唯尔有限公司出版。保留所有权利。
The diagnostic potential of recombinant Lepp12 (rLepp12) antigen cloned from Leishmania infantum was assessed in L. donovani infections by Western blotting. Ninety-two serum samples, including 30 patients with active kata-azar (KA), 17 post-treated KA patients (KA-PT), 20 post-kala-azar dermal leishmaniasis (PKDL) patients and 25 controls, were analysed for rLepp12, rK39 and DAT positivity. All KA samples taken at pre-treatment stage were positive for Lepp12 antibodies. Seventeen of these were evaluated post treatment (KA-PT), 10 of which were found to be negative. Nine of these 10 negative cases corresponded to clinically cured patients with regressed spleen. Seven post-treatment cases were rLepp12-positive; all of them corresponded to patients who were considered clinically cured but continued to have an enlarged spleen (>= 5 cm). The majority of PKDL patients (18/20) were found to be seronegative by immunoblot test using rLepp12 antigen. The rLepp12-based Western blot diagnosed 100% of patients with viscera[ disease, whilst none of the control cases were found to be reactive to rLepp12. rLepp12 protein provides a useful reagent for highly sensitive and specific diagnosis of KA. Additionally, rLepp12 appears to have potential as a prognostic marker for the infection. (c) 2007 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved.