Measurement of large distances in biomolecules using double-quantum filtered refocused electron spin-echoes
Measurement of large distances in biomolecules using double-quantum filtered refocused electron spin-echoes
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DOI：
10.1021/ja049372o
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发表时间：
2004-06-30
影响因子：
15
通讯作者：
Freed, JH
中科院分区：
文献类型：
--
作者：
Borbat, PP;Davis, JH;Freed, JH
它显示了如何双量子过滤重聚焦电子自旋回波的新技术是一个显着的改善双量子相干ESR，因为它增加了实验采集时间。这使得能够测量双标记生物分子中的较长距离。该方法是证明了一个长的双链A型RNA，自旋标记在两端。测量的72纳米的距离与分子模拟非常一致。
It is shown how the new technique of double-quantum filtered refocused electron spin−echoes is a significant improvement over double-quantum coherence ESR, since it increases the experimental acquisition time. This enables the measurement of longer distances in bilabeled biomolecules. The method is demonstrated on a long double-stranded A-type RNA, spin labeled at both ends. The measured distance of 72 Å is in excellent agreement with molecular modeling.