Changes in reflectin protein phosphorylation are associated with dynamic iridescence in squid
Changes in reflectin protein phosphorylation are associated with dynamic iridescence in squid
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DOI：
10.1098/rsif.2009.0299
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发表时间：
2010-03-06
影响因子：
3.9
通讯作者：
Morse, Daniel E.
中科院分区：
文献类型：
--
作者：
Izumi, Michi;Sweeney, Alison M.;Morse, Daniel E.
许多头足类动物都表现出显著的皮肤虹彩，这是它们复杂、动态伪装和交流的一个组成部分。在Euprymna scolope物种中，光器官的虹彩是静态的，这是由于基于反射素蛋白的血小板组装成称为虹膜小体的片状薄膜反射器，包含在称为虹膜细胞的虹膜细胞中。鱿鱼是独一无二的，其真皮具有动态的虹彩成分，具有反射的彩色结构，在M胆碱能系统和相关的神经递质乙酰胆碱(ACh)的控制下组装和分解。在这里，我们介绍了反射素家族的三个新成员的序列和特征，这些新成员与Lopolo中动态变化的虹膜相关，而静态的Euprymna虹膜载体中没有发现。此外，我们还发现应用蛋白酪氨酸激酶抑制剂金雀异黄素可以抑制ACh和钙诱导的LOGO虹彩形成。我们进一步证明，其中两个新的反射素被广泛的磷酸化，并与外源ACh激活虹彩相一致。这种磷酸化和相关的虹彩可以被金雀异黄素阻断。我们的结果表明，反射素蛋白的酪氨酸磷酸化参与了LOGO动态虹彩的调节。
Many cephalopods exhibit remarkable dermal iridescence, a component of their complex, dynamic camouflage and communication. In the species Euprymna scolopes, the light-organ iridescence is static and is due to reflectin protein-based platelets assembled into lamellar thin-film reflectors called iridosomes, contained within iridescent cells called iridocytes. Squid in the family Loliginidae appear to be unique in which the dermis possesses a dynamic iridescent component with reflective, coloured structures that are assembled and disassembled under the control of the muscarinic cholinergic system and the associated neurotransmitter acetylcholine (ACh). Here we present the sequences and characterization of three new members of the reflectin family associated with the dynamically changeable iridescence in Loligo and not found in static Euprymna iridophores. In addition, we show that application of genistein, a protein tyrosine kinase inhibitor, suppresses ACh- and calcium-induced iridescence in Loligo. We further demonstrate that two of these novel reflectins are extensively phosphorylated in concert with the activation of iridescence by exogenous ACh. This phosphorylation and the correlated iridescence can be blocked with genistein. Our results suggest that tyrosine phosphorylation of reflectin proteins is involved in the regulation of dynamic iridescence in Loligo.