Rapid maxillary expansion in alveolar cleft repaired with a tissue-engineered bone in a canine model
Rapid maxillary expansion in alveolar cleft repaired with a tissue-engineered bone in a canine model
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在犬模型中用组织工程骨修复牙槽裂的上颌骨快速扩张。
DOI：
10.1016/j.jmbbm.2015.03.029
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发表时间：
2015-08-01
影响因子：
3.9
通讯作者：
Qian, Yufen
中科院分区：
文献类型：
--
作者：
Huang, Jialiang;Tian, Bo;Qian, Yufen
本研究旨在探讨正畸扩弓对组织工程骨（TEB）BMSCs/β-TCP移植面积的影响，为牙槽突裂的治疗提供一种新的方法。建立单侧牙槽突裂犬模型，在快速上颌扩弓（RME）的条件下，应用BMSCs/β-TCP进行治疗。连续荧光标记，X线摄影和螺旋计算机断层扫描被用来评估新骨形成和矿化的移植区。苏木精-伊红染色和货车Gieson's picro fuchsin染色进行组织学和组织形态计量学观察。在成骨培养液中培养的BMSCs在β-TCP支架上生长，ALP活性、矿化及成骨分化相关基因的表达均得到促进。在此基础上，建立了TEB。与未治疗的狗或仅用自体髂骨治疗的狗相比，用BMSCs/β-TCP随后进行RME治疗8周后，新骨形成和矿化显著加速，骨吸收显著减少。TEE和RME联合治疗明显使骨小梁丰富，成骨面积增大。BMSCs/beta-TCP组与自体骨+RME组相比无显著性差异。本研究进一步揭示了TEB不仅是治疗牙槽突裂的一种可行的临床方法，而且是一种潜在的自体骨替代物，其与RME的结合可能是治疗牙槽突裂的一种替代策略。(C)2015爱思唯尔有限公司版权所有。
This study aims to investigate the effects of orthodontic expansion on graft area of a tissue-engineered bone (TEB) BMSCs/beta-TCP, and to find an alternative strategy for the therapy of alveolar cleft. A unilateral alveolar cleft canine model was established and then treated with BMSCs/beta-TCP under rapid maxillary expansion (RME). Sequential fluorescent labeling, radiography and helical computed tomography were used to evaluate new bone formation and mineralization in the graft area. Hematoxylin-eosin staining and Van Gieson's picro fuchsin staining were performed for histological and histomorphometric observation. ALP activity, mineralization and the expression of osteogenic differentiation related genes of BMSCs that grew on the beta-TCP scaffo(l)d were promoted by their cultivation in osteogenic medium. Based on fact, TEB was constructed. After 8 weeks of treatment with BMSCs/beta-TCP followed by RME, new bone formation and mineralization of the dogs were markedly accelerated, and bone resorption was significantly reduced, compared with the untreated dogs, or those only treated with autogenous iliac bone. The treatment with both TEE and RME evidently made the bone trabecula more abundant and the area of bone formation larger. What is more, there were no significant differences between BMSCs/beta-TCP group and the group treated with autogenous bone and RME. This study further revealed that TEB was not only a feasible clinical approach for patients with alveolar cleft, but also a potential substituent of autogenous bone, and its combination with RME might be an alternative strategy for the therapy of alveolar cleft. (C) 2015 Elsevier Ltd. All rights reserved.