Identification of serum biomarkers for necrotizing enterocolitis using aptamer-based proteomics.
Identification of serum biomarkers for necrotizing enterocolitis using aptamer-based proteomics.
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DOI：
10.3389/fped.2023.1184940
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发表时间：
2023
影响因子：
2.6
通讯作者：
中科院分区：
文献类型：
--
作者：
坏死性小肠结肠炎（NEC）是一种潜在的致命性肠道疾病，主要影响早产儿。早期诊断新生儿NEC对于改善预后至关重要;然而，传统的诊断工具仍然不足。生物标志物代表了提高诊断速度和准确性的机会，但它们并没有在临床实践中常规使用。在这项研究中，我们利用基于适体的蛋白质组学发现分析，以确定新的NEC的血清生物标志物。我们比较了新生儿NEC和非NEC的血清蛋白水平，并确定了这些组之间的10个差异表达的血清蛋白。我们检测到两种蛋白质，C-C基序趋化因子配体16（CCL 16）和免疫球蛋白重链恒定区α 1和2异二聚体（IGHA 1 IGHA 2），在NEC期间显着增加，8个显着减少。生成的受试者工作特征（ROC）曲线显示甲胎蛋白（AUC = 0.926）、胰高血糖素（AUC = 0.860）和IGHA 1 IGHA 2（AUC = 0.826）是最能区分NEC患者和非NEC患者的蛋白质。这些发现表明，进一步调查这些血清蛋白作为NEC的生物标志物是必要的。将来，结合这些差异表达蛋白的实验室检测可能会提高临床医生快速准确诊断NEC婴儿的能力。研究概述。图由Biorender.com制作。
Necrotizing enterocolitis (NEC) is a potentially fatal intestinal disease primarily affecting preterm infants. Early diagnosis of neonates with NEC is crucial to improving outcomes; however, traditional diagnostic tools remain inadequate. Biomarkers represent an opportunity to improve the speed and accuracy of diagnosis, but they are not routinely used in clinical practice. In this study, we utilized an aptamer-based proteomic discovery assay to identify new serum biomarkers of NEC. We compared levels of serum proteins in neonates with and without NEC and identified ten differentially expressed serum proteins between these groups. We detected two proteins, C-C motif chemokine ligand 16 (CCL16) and immunoglobulin heavy constant alpha 1 and 2 heterodimer (IGHA1 IGHA2), that were significantly increased during NEC and eight that were significantly decreased. Generation of receiver operating characteristic (ROC) curves revealed that alpha-fetoprotein (AUC = 0.926), glucagon (AUC = 0.860), and IGHA1 IGHA2 (AUC = 0.826) were the proteins that best differentiated patients with and without NEC. These findings indicate that further investigation into these serum proteins as a biomarker for NEC is warranted. In the future, laboratory tests incorporating these differentially expressed proteins may improve the ability of clinicians to diagnose infants with NEC rapidly and accurately. Overview of the study. Figure made with Biorender.com.
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影响因子：
3.7
作者：
Gold L;Ayers D;Bertino J;Bock C;Bock A;Brody EN;Carter J;Dalby AB;Eaton BE;Fitzwater T;Flather D;Forbes A;Foreman T;Fowler C;Gawande B;Goss M;Gunn M;Gupta S;Halladay D;Heil J;Heilig J;Hicke B;Husar G;Janjic N;Jarvis T;Jennings S;Katilius E;Keeney TR;Kim N;Koch TH;Kraemer S;Kroiss L;Le N;Levine D;Lindsey W;Lollo B;Mayfield W;Mehan M;Mehler R;Nelson SK;Nelson M;Nieuwlandt D;Nikrad M;Ochsner U;Ostroff RM;Otis M;Parker T;Pietrasiewicz S;Resnicow DI;Rohloff J;Sanders G;Sattin S;Schneider D;Singer B;Stanton M;Sterkel A;Stewart A;Stratford S;Vaught JD;Vrkljan M;Walker JJ;Watrobka M;Waugh S;Weiss A;Wilcox SK;Wolfson A;Wolk SK;Zhang C;Zichi D
通讯作者：
Zichi D
影响因子：
3
作者：
Nakame, Kazuhiko;Kaji, Tatsuru;Matsufuji, Hiroshi
通讯作者：
Matsufuji, Hiroshi
影响因子：
3.8
作者：
Mehan, Michael R.;Williams, Stephen A.;Ostroff, Rachel M.
通讯作者：
Ostroff, Rachel M.
影响因子：
4.6
作者：
Bogie, Jeroen F. J.;Mailleux, Jo;Hendriks, Jerome J. A.
通讯作者：
Hendriks, Jerome J. A.
DOI：
10.1073/pnas.1507719112
发表时间：
2015-06-09
影响因子：
11.1
作者：
Hathout Y;Brody E;Clemens PR;Cripe L;DeLisle RK;Furlong P;Gordish-Dressman H;Hache L;Henricson E;Hoffman EP;Kobayashi YM;Lorts A;Mah JK;McDonald C;Mehler B;Nelson S;Nikrad M;Singer B;Steele F;Sterling D;Sweeney HL;Williams S;Gold L
通讯作者：
Gold L