Increases in matrix metalloproteinase‐9 and tissue inhibitor of matrix metalloproteinase‐1 mRNA after cerebral contusion and depolarisation
Increases in matrix metalloproteinase‐9 and tissue inhibitor of matrix metalloproteinase‐1 mRNA after cerebral contusion and depolarisation
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脑挫裂伤和去极化后基质金属蛋白酶 9 和基质金属蛋白酶组织抑制剂 1 mRNA 增加
DOI：
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发表时间：
2003
影响因子：
4.2
通讯作者：
A. S. Sandberg Nordqvist
中科院分区：
文献类型：
--
作者：
C. von Gertten;S. Holmin;T. Mathiesen;A. S. Sandberg Nordqvist
Matrix metalloproteinases (MMPs) and tissue inhibitors of matrix metalloproteinases (TIMPs) play major roles in physiological extracellular matrix turnover during normal development and in pathological processes. In brain, increases in MMP activity occur, for example, in multiple sclerosis, Alzheimer's disease, and after head trauma. We examined MMP‐9 and TIMP‐1, ‐2, and ‐3 in events after head trauma. A time‐course study was carried out using two different rat injury models, cerebral contusion and depolarisation. Brains were analysed by RT‐PCR and in situ hybridisation. We observed a distinct and time‐dependent upregulation of MMP‐9 and TIMP‐1 mRNA in ipsilateral cortical areas. MMP‐9 mRNA levels were upregulated 1 day after cerebral contusion with a peak at Day 4. Depolarisation per se, which also occurs after traumatic brain injury, lead to delayed increase of MMP‐9 mRNA, 4 days post application. At Day 14, MMP‐9 mRNA levels were indistinguishable from controls in both models. TIMP‐1 mRNA increases were observed in both models 4 hr after injury, and increased further at Days 1 and 4. At Day 14, mRNA levels declined and were no higher than control levels. No alterations in mRNA levels were noted for TIMP‐2 or ‐3. Our results support earlier reports on MMP‐9 involvement in brain injury. It also shows a role for TIMP‐1 in the mechanisms of trauma, where depolarisation could be the mechanism responsible for this upregulation. © 2003 Wiley‐Liss, Inc.
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DOI：
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发表时间：
1998-03
期刊：
The American journal of pathology
影响因子：
--
作者：
Axel Pagenstecher;Anne K. Stalder;C. Kincaid;Steven D. Shapiro;lain L. Campbell
通讯作者：
Axel Pagenstecher;Anne K. Stalder;C. Kincaid;Steven D. Shapiro;lain L. Campbell
影响因子：
4.2
作者：
Mori, T;Wang, XY;Lo, EH
通讯作者：
Lo, EH
影响因子：
8.3
作者：
Rosenberg, GA;Estrada, EY;Dencoff, JE
通讯作者：
Dencoff, JE
DOI：
10.1016/0169-328x(95)00289-5
发表时间：
1996-04-01
期刊：
MOLECULAR BRAIN RESEARCH
影响因子：
--
作者：
Raghupathi, R;McIntosh, TK
通讯作者：
McIntosh, TK