THE FINE STRUCTURE OF TESTICULAR INTERSTITIAL CELLS IN GUINEA PIGS
THE FINE STRUCTURE OF TESTICULAR INTERSTITIAL CELLS IN GUINEA PIGS
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豚鼠睾丸间质细胞的精细结构
DOI：
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发表时间：
1965
影响因子：
7.8
通讯作者：
A. Christensen
中科院分区：
文献类型：
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作者：
A. Christensen
在用戊二醛或四氧化锇固定剂灌注的豚鼠睾丸中，间质细胞的细胞质含有异常丰富的无颗粒内质网。在细胞中心区域的网状物是相互连接的小管的网络，但在广泛的外围区域，网状物通常被组织成紧密排列的扁平的有孔池。偶尔在细胞质中出现小片的颗粒网，并与无颗粒网自由连接。线粒体具有致密的基质，并含有嵴和一些小管。高尔基复合体是分散的，没有分泌物质的证据。细胞质也含有脂滴。脂褐素色素颗粒可能是多形态残留体，含有三种成分：（1）在高倍放大下显示75-A周期性的致密物质;（2）中等大小的脂滴;和（3）帽状结构。在戊二酸二钠灌注的睾丸中，间质细胞的细胞质似乎在细胞与细胞之间具有相同的密度，无颗粒网是管状或池状的，但不是空泡的形式。因此，有时在其他固定中遇到的“暗”和“亮”细胞以及囊状无颗粒网状物可能是伪影。与本研究结果相关的其他实验室的生化结果表明，豚鼠间质细胞中的无颗粒内质网膜是雄激素生物合成的至少两种酶（17-羟化酶和17-碳链酶）的位点。
In guinea pig testes perfused with either glutaraldehyde or osmium tetroxide fixative, the cytoplasm of the interstitial cells contains an exceptionally abundant agranular endoplasmic reticulum. The reticulum in central regions of the cell is a network of interconnected tubules, but in extensive peripheral areas the reticulum is commonly organized into closely packed, flattened cisternae which are fenestrated. Occasional small patches of the granular reticulum occur in the cytoplasm and connect freely with the agranular reticulum. The mitochondria have a dense matrix and contain cristae and some tubules. The Golgi complex is disperse and shows no evidence of secretory material. The cytoplasm also contains lipid droplets. Lipofuscin pigment granules are probably polymorphic residual bodies and contain three components: (1) a dense material which at high magnification shows a 75-A periodicity; (2) a medium-sized lipid droplet; and (3) a cap-like structure. In glutaraldehyde-perfused testis the interstitial cell cytoplasm appears to have the same density from cell to cell, and the agranular reticulum is tubular or cisternal but not in the form of empty vesicles. Thus the "dark" and "light" cells and the vesicular agranular reticulum sometimes encountered in other fixations may be artifacts. Biochemical results from other laboratories, correlated with the present findings, indicate that the membranes of the agranular endoplasmic reticulum in guinea pig interstitial cells are the site of at least two enzymes of androgen biosynthesis, the 17-hydroxylase and the 17-desmolase.