Residualization Rates of Near-Infrared Dyes for the Rational Design of Molecular Imaging Agents.
Residualization Rates of Near-Infrared Dyes for the Rational Design of Molecular Imaging Agents.
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DOI：
10.1007/s11307-015-0851-7
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发表时间：
2015-12
影响因子：
3.1
通讯作者：
Thurber GM
中科院分区：
文献类型：
--
作者：
Cilliers C;Liao J;Atangcho L;Thurber GM
近红外（NIR）荧光成像被广泛用于跟踪体内的抗体和生物分子。临床和临床前应用包括术中成像、追踪治疗和荧光标记作为后续放射性标记的替代。尽管它们被广泛使用，但NIR染料的基本性质之一，内化后细胞内的残留率，尚未被系统地研究。该速率是合理设计探针和正确解释体内结果所必需的。在这份简短的报告中，我们测量了8种常用染料的细胞残留率，这些染料包括三种核心结构（花青，BODIPY和恶嗪/噻嗪/carbopyronin）。我们鉴定了在远红（~650-680 nm）和近红外（~740-800 nm）区域中的残留（半衰期> 24小时）和非残留染料（半衰期< 24小时）。这些数据将使研究人员能够独立合理地选择分子成像剂设计的染料的波长和残留性质。
Near infrared (NIR) fluorescence imaging is widely used for tracking antibodies and biomolecules in vivo. Clinical and preclinical applications include intraoperative imaging, tracking therapeutics, and fluorescent labeling as a surrogate for subsequent radiolabeling. Despite their extensive use, one of the fundamental properties of NIR dyes, the residualization rate within cells following internalization, has not been systematically studied. This rate is required for the rational design of probes and proper interpretation of in vivo results. In this brief report, we measure the cellular residualization rate of eight commonly used dyes encompassing three core structures (cyanine, BODIPY, and oxazine/thiazine/carbopyronin). We identify residualizing (half-life > 24 hrs) and non-residualizing dyes (half-life < 24 hrs) in both the far red (~650-680 nm) and near infrared (~740-800 nm) regions. This data will allow researchers to independently and rationally select the wavelength and residualizing nature of dyes for molecular imaging agent design.