Transcriptome de novo assembly and analysis of differentially expressed genes related to cytoplasmic male sterility in onion.
Transcriptome de novo assembly and analysis of differentially expressed genes related to cytoplasmic male sterility in onion.
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DOI：
10.1016/j.plaphy.2018.01.015
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发表时间：
2018-04
期刊：
影响因子：
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通讯作者：
Qiaoling Yuan;Ce Song;Luyao Gao;Huihui Zhang;Cuicui Yang;Jie Sheng;Jian Ren;Dian Chen;Yong Wang
中科院分区：
文献类型：
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作者：
Qiaoling Yuan;Ce Song;Luyao Gao;Huihui Zhang;Cuicui Yang;Jie Sheng;Jian Ren;Dian Chen;Yong Wang
洋葱(Allium Cepal.)是中国的主要蔬菜之一，占中国蔬菜出口的很大比例。洋葱细胞质雄性不育是洋葱核基因与细胞质相互作用的结果，是洋葱育种中常用的雄性不育类型。然而，洋葱细胞质雄性不育的潜在分子机制仍不清楚。本研究对洋葱细胞质雄性不育系SA2和保持系SB2的花药显微结构进行了分析。我们发现，SA2的花粉败育发生在小孢子发育的四分体阶段，这与SB2有很大的不同。我们使用Illumina HiSeq平台对从SA2和SB2品系收集的四分体阶段的花药RNA进行了测序。RNA测序和转录组组件产生了146,413个全非基因组。通过对差异表达基因的分析，我们发现了两个细胞质控制基因atp9和cox1，以及三个与核相关的基因SERK1、AG和AMS。这些转录结果也被荧光定量聚合酶链式反应所证实。本研究为洋葱细胞质雄性不育相关基因的研究提供了重要信息，有助于进一步了解洋葱细胞质雄性不育的分子机制。
Onion (Allium cepaL.) is one of the major vegetables in China and accounts for a large proportion of China's vegetable exports. Onion cytoplasmic male sterility, which is often used in onion breeding, is caused by the interaction between the nuclear genes and the cytoplasm. However, the underlying molecular mechanism of onion cytoplasmic male sterility remains unclear. In this study, we analysed the anther microstructure of the onion cytoplasmic male sterile line SA2 and the onion maintainer line SB2. We found that the pollen abortion in SA2 occurred at the tetrad stage during the microspore development, which was very different from that in SB2. We used the Illumina HiSeq platform to sequence RNA from anthers at the tetrad stage collected from the SA2 and SB2 lines. The RNA sequencing and transcriptome assembly produced 146,413 All-Unigenes. Based on an analysis of the differentially expressed genes, we identified two cytoplasmic control genes,atp9andcox1, and three nuclear-related genes,SERK1,AGandAMS. These transcriptomic results were also verified by fluorescence quantitative PCR. Our study provides important information about genes related to onion cytoplasmic male sterility, and it will help improve the understanding of the molecular mechanism of onion cytoplasmic male sterility.