A WD repeat protein, Rec14, essential for meiotic recombination in Schizosaccharomyces pombe.
A WD repeat protein, Rec14, essential for meiotic recombination in Schizosaccharomyces pombe.
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发表时间：
1997-08
期刊：
影响因子：
3.3
通讯作者：
David R.H Evans;Ywan Feng Li;M. Fox;Gerald R. Smith
中科院分区：
文献类型：
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作者：
David R.H Evans;Ywan Feng Li;M. Fox;Gerald R. Smith
Pombe裂殖酵母rec14基因的突变使减数分裂重组减少了1000倍。遗传和物理分析表明，与rec14基因突变互补的DNA克隆含有rec14基因，该基因在小至1.4kb的质粒型插入片段中起作用。通过对rec14转录本的分析，证实了rec14基因含有两个外显子，外显子之间由一个53-bp的内含子隔开。剪接的转录本编码一个由302个氨基酸组成的蛋白质产物，其中包含6个WD重复基序，发现于G-β转导蛋白家族和其他蛋白质中，包括酿酒酵母Ski8(Rec103)蛋白质。虽然rec14转录本存在于有丝分裂细胞中，但rec14突变对有丝分裂重组没有可检测到的影响。Rec14的表达模式不同于先前分析的S.pombe rec基因。根据突变的表型和氨基酸序列的相似性，我们推测S.pombe Rec14是S.cerevisiae Rec103的功能同源物。
Mutations in the Schizosaccharomyces pombe rec14 gene reduce meiotic recombination by as much as a factor of 1000 in the three intervals tested on chromosomes I and III. A DNA clone complementing the rec14 mutation was shown by genetic and physical analysis to contain the rec14 gene, which was functional in plasmid-borne inserts as small as 1.4 kb. The rec14 gene contains two exons separated by a 53-bp intron, which was confirmed by analysis of rec14 transcripts. The spliced transcript encodes a protein product of 302 amino acids, which contains six WD repeat motifs found in the G-beta transducin family of proteins and other proteins, including the Saccharomyces cerevisiae Ski8 (Rec103) protein. Although the rec14 transcripts were present in mitotically dividing cells, rec14 mutations had no detectable effect on mitotic recombination. The pattern of expression of rec14 differes from that of previously analyzed S. pombe rec genes. Based upon mutant phenotypes and amino acid sequence similarities, we propose that S. pombe Rec14 is a functional homologue of S. cerevisiae Rec103.