Extracellular polymeric substances affect the responses of multi-species biofilms in the presence of sulfamethizole.
Extracellular polymeric substances affect the responses of multi-species biofilms in the presence of sulfamethizole.
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DOI：
10.1016/j.envpol.2017.12.060
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发表时间：
2018-04
影响因子：
8.9
通讯作者：
Longfei Wang;Yi Li;Li Wang;Huanjun Zhang;Mengjie Zhu;Pei-Yi Zhang;Xiaoxiao Zhu
中科院分区：
文献类型：
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作者：
Longfei Wang;Yi Li;Li Wang;Huanjun Zhang;Mengjie Zhu;Pei-Yi Zhang;Xiaoxiao Zhu
抗生素在天然和工程生物膜中的发生和运输引起了人们越来越多的关注。然而，细胞外聚合物(EPS)对生物膜对抗生素反应的影响尚不清楚。在这项研究中，研究了EPS对典型抗生素磺胺二甲基咪唑(STZ)在模型生物膜中的吸附和生物反应的影响。EPS与STZ之间的相互作用以蛋白质为主，移动床生物膜反应器中的EPS与STZ的相互作用最强。EPS是STZ的重要储库，提取EPS后，所测试的生物膜对STZ的吸附能力都有所降低。EPS提取后，呼吸频率和典型酶活性均降低。高通量16S rRNA基因测序结果证实，没有EPS的生物膜中的细菌群落更容易受到抗生素休克的影响，群落多样性和丰富度指数表明。在自然生物膜中观察到敏感物种的丰度有较大的增加。结果表明，EPS在STZ的生物吸附中发挥了重要作用，减轻了抗生素对细胞的直接损伤；此外，细菌群落反应的程度与生物膜的来源有关。我们的研究提供了多物种生物膜对抗生素暴露的反应的详细信息，并强调了EPS在与抗生素相互作用中的作用，从而加深了对现实生活中自然和工程生物膜系统中抗生素生物修复的理解。
The occurrence and transportation of antibiotics in biofilms from natural and engineered sources have attracted increasing interests. Nevertheless, the effects of extracellular polymeric substances (EPS) on the responses of biofilms to the exposure to antibiotics are not clear. In this study, the effects of EPS on the sorption and biological responses to one representative antibiotic, sulfamethizole (STZ), in model biofilms were investigated. Proteins dominated the interactions between the EPS and the STZ and the EPS from a moving bed biofilm reactor exhibited the strongest interaction with the STZ. The EPS served as important reservoirs for the STZ and the tested biofilms all showed reduced sorption capacities for the STZ after the EPS were extracted. The respiratory rates and typical enzymatic activities were reduced after the EPS were extracted. High-throughput 16S rRNA gene sequencing results confirmed that the bacterial community in the biofilm without the EPS was more vulnerable to antibiotic shock as indicated by the community diversity and richness indices. A greater increase in the abundance of susceptible species was observed in the natural biofilm. The results comprehensively suggested that the EPS played important role in biosorption of STZ and alleviated the direct damage of the antibiotic to the cells; in addition the extent of the bacterial community response was associated with the origins of the biofilms. Our study provided details on the responses of multi-species biofilms to the exposure to an antibiotic and highlighted the role of the EPS in interacting with the antibiotic, thereby providing a deeper understanding of the bioremediation of antibiotics in real-life natural and engineered biofilm systems.