Osteogenic Potential of Multipotent Adult Progenitor Cells for Calvaria Bone Regeneration.
Osteogenic Potential of Multipotent Adult Progenitor Cells for Calvaria Bone Regeneration.
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DOI：
10.1155/2016/2803081
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发表时间：
2016
影响因子：
--
通讯作者：
Ko CC
中科院分区：
文献类型：
--
作者：
Lee DJ;Park Y;Hu WS;Ko CC
研究了大鼠多能成体祖细胞（rMAPCs）来源的成骨细胞在骨再生中的潜在用途。rMAPCs是来源于骨髓的成体干细胞，具有高增殖能力和向多个谱系分化的潜力。它们还可以提供有利于再生医学应用的免疫调节特性。将rMAPCs作为单细胞或作为3D聚集体在成骨培养基中培养长达38天，然后通过骨钙素和I型胶原的免疫染色和矿化测定来评估它们向骨谱系的分化。通过在大鼠颅骨缺损中直接植入多能成体祖细胞（MAPC）聚集体，在体内评价rMAPC促进骨再生的能力。骨再生检查放射学，组织学和组织形态计量学。结果表明，rMAPC成功地分化为成骨细胞系，证明矿化的细胞外基质形成在体外和诱导新骨形成的rMAPC聚集体的作用在体内。这些结果证实了rMAPCs具有良好的成骨潜力，并提供了rMAPCs作为骨再生的新型成体干细胞来源的见解。
Osteogenic cells derived from rat multipotent adult progenitor cells (rMAPCs) were investigated for their potential use in bone regeneration. rMAPCs are adult stem cells derived from bone marrow that have a high proliferation capacity and the differentiation potential to multiple lineages. They may also offer immunomodulatory properties favorable for applications for regenerative medicine. rMAPCs were cultivated as single cells or as 3D aggregates in osteogenic media for up to 38 days, and their differentiation to bone lineage was then assessed by immunostaining of osteocalcin and collagen type I and by mineralization assays. The capability of rMAPCs in facilitating bone regeneration was evaluated in vivo by the direct implantation of multipotent adult progenitor cell (MAPC) aggregates in rat calvarial defects. Bone regeneration was examined radiographically, histologically, and histomorphometrically. Results showed that rMAPCs successfully differentiated into osteogenic lineage by demonstrating mineralized extracellular matrix formation in vitro and induced new bone formation by the effect of rMAPC aggregates in vivo. These outcomes confirm that rMAPCs have a good osteogenic potential and provide insights into rMAPCs as a novel adult stem cell source for bone regeneration.