Association of syndecan-1 with tumor grade and histology in primary invasive cervical carcinoma
Association of syndecan-1 with tumor grade and histology in primary invasive cervical carcinoma
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DOI：
10.1006/gyno.1999.5595
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发表时间：
1999-12-01
影响因子：
4.7
通讯作者：
Grénman, S
中科院分区：
文献类型：
--
作者：
Rintala, M;Inki, P;Grénman, S
目标。Syndecan-1是一种细胞表面硫酸乙酰肝素蛋白多糖，在鳞状细胞恶性转化过程中表达减少。对头颈部鳞状细胞癌的研究表明，Syndecan-1阳性的肿瘤与更长的总体和无复发的生存期有关。本研究的目的是分析Syndecan-1在浸润性宫颈癌中的表达，并探讨Syndecan-1的表达与预后因素和总生存期的关系。研究人群包括在1970-1988年间在图尔库大学中心医院接受治疗的124名原发性浸润性宫颈癌患者。其中鳞癌102例(82.3%)，腺癌16例(12.9%)，腺鳞癌1例(0.8%)，小细胞癌1例(0.8%)，腺样基底细胞癌1例(0.8%)，癌肉瘤1例(0.8%)，未分类宫颈癌2例(1.6%)。用人类Syndecan-1特异性单抗B-B4和免疫组织化学方法检测石蜡包埋组织块上Syndecan-1的表达。根据染色强度和阳性肿瘤细胞百分率对Syndecan-1的表达进行分类。44例(36%)标本染色较强，24例(19%)syndecan-1阴性。在49例(40%)标本中，Syndecan-1阳性细胞的百分比大于或等于90%。Syndecan-1的表达与肿瘤分化程度(P=0.001.0 3)和鳞状组织学(P<；0.0 5)有关，与临床分期(P=0.16)或无瘤生存期(P=0.86)无关。年龄(P=0.003)和临床分期(P<；0.001)是显著的预后因素，但Syndecan-1的表达与预后无关，既不取决于阳性肿瘤细胞的百分比，也不取决于染色强度。在宫颈癌中，Syndecan-1与组织分化程度和鳞状组织学有关，但不能预测临床结果。(C)1999年学术出版社。
Objectives. The expression of syndecan-1, a cell surface heparan sulfate proteoglycan, is reduced during malignant transformation of squamous cells. Studies on squamous cell carcinoma of the head and neck have shown that syndecan-1-positive tumors are associated with longer overall and recurrence-free survival. The purpose of this study was to analyze syndecan-1 expression in invasive cervical carcinoma and to examine the association of syndecan-1 expression with prognostic factors and overall survival.Methods. The study population consisted of 124 patients treated for primary invasive carcinoma of the uterine cervix at the Turku University Central Hospital during the years 1970-1988. The material consisted of 102 (82.3%) squamous cell carcinomas, 16 (12.9%) adenocarcinomas and 1 (0.8%) adenosquamous carcinoma, 1(0.8%) small cell carcinoma, 1 (0.8%) adenoid basal carcinoma, 1 (0.8%) carcinosarcoma, and 2 (1.6%) unclassified cervical carcinomas. Syndecan-1 expression was determined on paraffin-embedded tissue blocks using a human syndecan-1-specific monoclonal antibody B-B4 and immunohistochemistry. The expression of syndecan-1 was classified according to staining intensity as well as the percentage of positively stained tumor cells.Results. Staining intensity was strong in 44 (36%) samples, while 24 (19%) specimens remained syndecan-1-negative. In 49 (40%) samples, the percentage of syndecan-1-positive cells was greater than or equal to 90%. Syndecan-1 expression, as determined by greater than or equal to 50% positively stained tumor cells, was associated with the grade of differentiation (P = 0.03) and squamous histology (P < 0.001), but was not associated with clinical stage (P = 0.16) or disease-free survival (P = 0.86). Age (P = 0.003) and clinical stage (P < 0.001) were significant prognostic factors, but syndecan-1 expression determined neither by percentage of positively stained tumor cells nor by staining intensity was associated with the outcome.Conclusions. In cervical carcinoma syndecan-1 is associated with histological differentiation grade and squamous histology, but does not predict clinical outcome. (C) 1999 Academic Press.