Continuous monitoring of oxygen concentrations in several systems for cultivation of anaerobic bacteria
Continuous monitoring of oxygen concentrations in several systems for cultivation of anaerobic bacteria
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DOI：
10.1128/jcm.34.7.1646-1648.1996
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发表时间：
1996-07-01
影响因子：
9.4
通讯作者：
Heinzer, I
中科院分区：
文献类型：
--
作者：
Imhof, A;Heinzer, I
厌氧室和罐是临床微生物实验室中用于产生厌氧气氛的两种常规方法。比较了抽空置换法、Oxoid AnaeroGen、Merck Anaerocult A、BBL GasPak、BBL GasPakPlus、亚当斯Scientific GasGendicator、Difco Anaerobic和bioMerieux Generbox anaer系统在厌氧罐中氧浓度的定时降低。每个系统重复实验10次。氧浓度用氧分析仪系列3600仪器（OrbischantLaboratories，Neuchatel-Geneva Switzerland）测量。BBL GasPak、BBL GasPakPlus、bioMerieux Generbox、亚当斯科学气体发生器和Difco厌氧系统含有硼氢化钠，可释放氢气。A系统含有铁粉，可以结合氧气。AnaeroGen系统的活性成分是抗坏血酸。达到0.5%的O-2浓度的时间为8至15分钟的抽空置换法，26至41分钟的AnaeroGen系统，60至93分钟的Anaerocult A系统，和22至419分钟的硼氢化钠系统。AnaeroGen系统，Anaerocult A系统和抽空置换法从未失败过产生厌氧气氛。硼氢化钠系统在70次运行中有10次失败。这些结果表明，应使用抽空置换法或Oxoid AnaeroGen系统来产生厌氧气氛。Anaerocult A系统显示出良好的再现性，但达到适当的低氧气水平所需的时间太长。由于故障率高，不推荐使用硼氢化物系统。
Anaerobic chambers and jars are the two conventional methods used in clinical microbiology laboratories to produce an anaerobic atmosphere. The evacuation-replacement method, the Oxoid AnaeroGen, the Merck Anaerocult A, the BBL GasPak, the BBL GasPakPlus, the Adams Scientific GasGendicator, the Difco Anaerobic, and the bioMerieux Generbox anaer systems were compared for the timed decrease in the oxygen concentration in an anaerobic jar. The experiment was repeated 10 times for each system. The oxygen concentration was measured with an oxygen analyzer series 3600 instrument (Orbisphere Laboratories, Neuchatel-Geneva Switzerland). The BBL GasPak, the BBL GasPakPlus, the bioMerieux Generbox, the Adams Scientific GasGendicator, and the Difco Anaerobic systems contain sodium borohydride, which liberates hydrogen. The Anaerocult A system contains iron powder which binds the oxygen. The active ingredient of the AnaeroGen system is ascorbic acid. The times to reach an O-2 concentration of 0.5% were 8 to 15 min for the evacuation-replacement method, 26 to 41 min for the AnaeroGen system, 60 to 93 min for the Anaerocult A system, and 22 to 419 min for the sodium borohydride systems. The AnaeroGen system, the Anaerocult A system, and the evacuation-replacement method never failed to produce an anaerobic atmosphere. The sodium borohydride systems failed in 10 of 70 runs. These results suggest that the evacuation-replacement method or the Oxoid AnaeroGen system should be used to produce an anaerobic atmosphere. The Anaerocult A system showed a good reproducibility, but the length of time required to reach an appropriately low level of oxygen was too long. Because of the high failure rate, the borohydride systems cannot be recommended.