THE HUMAN OX40 HOMOLOG - CDNA STRUCTURE, EXPRESSION AND CHROMOSOMAL ASSIGNMENT OF THE ACT35 ANTIGEN
THE HUMAN OX40 HOMOLOG - CDNA STRUCTURE, EXPRESSION AND CHROMOSOMAL ASSIGNMENT OF THE ACT35 ANTIGEN
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DOI：
10.1002/eji.1830240329
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发表时间：
1994-03-01
影响因子：
5.4
通讯作者：
STEIN, H
中科院分区：
文献类型：
--
作者：
LATZA, U;DURKOP, H;STEIN, H
组织分布以及在有丝分裂原和病毒刺激的淋巴细胞上的表达使得ACT35分子成为人类淋巴细胞活化抗原，但目前还不能对其进行聚类。来自 HUT-102 细胞系 pCDM8 文库的 ACT35 抗原的表达克隆揭示了 cDNA 及其编码的蛋白质序列与先前描述的大鼠 OX40 抗原的强同源性。单跨膜蛋白的 1.4 kb 核苷酸序列和推导的 277 个氨基酸序列在人和大鼠中分别有 65% 和 63% 的同一性。保守性包括 1 个 N 连接糖基化位点和 1 个蛋白激酶 C 磷酸化位点。当在 COS-1 细胞中表达时，cDNA 呈现出与天然 ACT35 抗原和大鼠 OX40 分子（相对分子质量 48 000）相当的特性。因此，ACT35蛋白对应于迄今为止未知的人类OX40抗原，因此是肿瘤坏死因子/神经生长因子受体(TNFR/NGFR)家族的另一个成员。应用荧光原位杂交后，人类 ACT35/OX40 基因可以定位到染色体带 1p36，从而与 TNFR II 和 CD30 基因连接。
Tissue distribution and expression on mitogen and virally stimulated lymphocytes render the ACT35 molecule a human lymphocyte activation antigen which as yet could not be clustered. Expression cloning of the ACT35 antigen from a pCDM8 library of the HUT-102 cell line revealed strong homology of the cDNA and its encoded protein sequence with the formerly described rat OX40 antigen. The 1.4-kb nucleotide sequence and the deduced 277-amino acid sequence of the single transmembrane protein were 65% and 63% identical, in human and in rat, respectively. Conservation included one N-linked glycosylation site and one protein kinase C phosphorylation site. When expressed in COS-1 cells, the cDNA presented properties comparable to the native ACT35 antigen and the rat OX40 molecule (relative molecular mass 48 000). Thus, the ACT35 protein corresponds to the hitherto unknown human OX40 antigen and is, therefore, another member of the tumor necrosis factor/nerve growth factor receptor (TNFR/NGFR) family. After applying fluorescence in situ hybridization, the human ACT35/OX40 gene could be mapped to chromosome band 1p36 and is, thus, linked to the genes for TNFR II and CD30.