Genetic fingerprinting of Flavobacterium columnare isolates from cultured fish
Genetic fingerprinting of Flavobacterium columnare isolates from cultured fish
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DOI：
10.1111/j.1365-2672.2004.02314.x
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发表时间：
2004-01-01
影响因子：
4
通讯作者：
Klesius, PH
中科院分区：
文献类型：
--
作者：
Arias, CR;Welker, TL;Klesius, PH
目的：评价鱼类病原菌柱状黄杆菌的种内多样性。用16S rDNA的限制性片段长度多态性分析、基因间隔区(ISR)测序和AFLP(R)指纹图谱对Columnare分离株进行了鉴定。佛罗里达州30号研究包括从不同鱼类物种和地理来源以及参考菌株分离的Columnare培养物。15个菌株属于基因变种I，11个属于基因变种II。ISR序列分析证实了这两个基因变种之间的遗传差异，但揭示了基因变种I之间的较高多样性。AFLP(R)指纹图谱的分辨率最高，因为种内可以定义多达22个AFLP图谱。结论：我们确认了F1的划分。将养殖鱼类的分离株归入不同的基因组。我们发现，来自美国的斑点叉尾鱼中都存在基因变种I和II。我们描述了一个由四个F1代表的独特的遗传群体。巴西罗非鱼的Columnare分离株似乎与这两个基因组都有亲缘关系。我们能够通过分析ISR进一步细分物种。最后，AFLP(R)的使用使我们能够在克隆水平上对物种进行指纹识别，而不会失去基因组变异的更高遗传等级。研究的意义和影响：本文报告了对使用分子工具研究鱼类病原体F1的广泛评估。哥伦纳尔。
Aims: To evaluate the intraspecific diversity of the fish pathogen Flavobacterium columnareMethods and Results: Genetic variability among Fl. columnare isolates was characterized using restriction fragment length polymorphism analysis of the 16S rDNA gene, intergenic spacer region (ISR) sequencing, and amplified fragment length polymorphism (AFLP(R)) fingerprinting. Thirty Fl. columnare cultures isolated from different fish species and geographical origins as well as reference strains were included in the study. Fifteen isolates belonged to genomovar I while eleven were ascribed to genomovar II. Analysis of the ISR sequence confirmed the genetic differences between both genomovars but revealed a higher diversity among genomovar I isolates. The maximum resolution was provided by AFLP(R) fingerprinting, as up to 22 AFLP profiles could be defined within the species.Conclusions: We confirmed the division of Fl. columnare isolates from cultured fish into different genogroups. We showed that both genomovars I and II are present in channel catfish from the US. We described a unique genetic group represented by four Fl. columnare isolates from tilapia in Brazil which appears to be related to both genomovars. We were able to further subdivide the species by analysing the ISR. Finally, the use of AFLP(R) allowed us to fingerprint the species at clone level without losing the higher genetic hierarchy of genomovar division.Significance and Impact of the Study: This paper reports on an extensive assessment of the use of molecular tools for the study of the epidemiology of the fish pathogen Fl. columnare.