Conservation of a novel protein associated with an antibiotic efflux operon in Burkholderia cenocepacia
Conservation of a novel protein associated with an antibiotic efflux operon in Burkholderia cenocepacia
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DOI：
10.1016/j.femsle.2005.03.027
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发表时间：
2005-04-15
影响因子：
2.1
通讯作者：
Burns, JL
中科院分区：
文献类型：
--
作者：
Nair, BM;Joachimiak, LA;Burns, JL
洋葱洋葱伯克霍尔德菌是囊性纤维化患者的一个重要问题，是一种密切相关的耐药细菌洋葱洋葱杆菌复合体的成员。一种水杨酸调节的抗生素外排操纵子已经在cenocepacia杆菌中被发现，它的四个基因之一，llpE，在以前报道的外排操纵子中是没有平行的。从洋葱芽孢杆菌复合体分离物中扩增和测序的结果表明，洋葱芽孢杆菌中llpE的流行率最高，且序列高度保守。虽然在不同基因组变体的分离株之间至少鉴定出一个非同义突变，但在B. cenocepacia的IIIA和IIIB亚群中仅鉴定出同义突变。结构模型表明L1pE是α / β水解酶家族的成员。在cenocepacia中鉴定出与水解酶的强结构同源性和高度保守性，表明酶对L1pE有作用，有利于囊性纤维化肺的生存。(c) 2005年欧洲微生物学会联合会。Elsevier B.V.版权所有。
Burkholderia cenocepacia is a significant problem in individuals with cystic fibrosis and is a member of the B. cepacia complex of closely related antibiotic resistant bacteria. A salicylate-regulated antibiotic efflux operon has been identified in B. cenocepacia and one of its four genes, llpE, is without parallel in previously reported efflux operons. PCR amplification and sequencing of llpE from B. cepacia complex isolates demonstrated the highest prevalence in B. cenocepacia with a high degree of sequence conservation. While at least one non-synonymous mutation was identified between isolates from different genomovars, only synonymous differences were identified within the IIIA and IIIB sub-groups of B. cenocepacia. Structural modeling suggests that L1pE is a member of the alpha/beta hydrolase enzyme family. Identification of strong structural homology to hydrolases and a high degree of conservation in B. cenocepacia suggests an enzymatic function for L1pE, benefiting survival in the cystic fibrosis lung. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.