Pathogenesis studies with Australian bat lyssavirus in grey-headed flying foxes (Pteropus poliocephalus)
Pathogenesis studies with Australian bat lyssavirus in grey-headed flying foxes (Pteropus poliocephalus)
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DOI：
10.1111/j.1751-0813.2002.tb10973.x
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发表时间：
2002-10-01
影响因子：
1.1
通讯作者：
Westbury, HA
中科院分区：
文献类型：
--
作者：
McColl, KA;Chamberlain, T;Westbury, HA
目的检测灰头狐蝠（Pteropus poliocephalus）对澳大利亚蝙蝠狂犬病病毒（ABL）的敏感性，并对该病的发病机制进行初步观察。每天观察接种动物，每9至14天采集临床样本。对出现异常临床体征的动物实施安乐死，并采集样本进行组织学、血清学、病毒学和免疫组织学检查。在3个月后接种（PI），所有的幸存者被安乐死，并提交了一个类似的examination.Results三ABL接种的狐蝠，和两个狂犬病接种的动物发展异常的临床症状之间的15和24天P1。所有三只ABL接种动物的组织学病变与狂犬病毒感染一致，并且在每只动物的中枢神经系统（CNS）中鉴定出狂犬病毒抗原。从两只受感染动物的脑中分离出病毒。狂犬病接种的飞狐，都有组织学病变和病毒抗原在中枢神经系统。只有一个人的大脑里发现了病毒。五只受影响的狐蝠都没有产生抗狂犬病病毒抗体，但是，在P1第3个月，七只接种ABL的幸存者中有五只，两只接种狂犬病病毒的幸存者中有一只发生了血清转化。动态的免疫反应是相当variable.Conclusions响应的飞狐ABL，管理的外周接种途径，是类似的蝙蝠外周接种蝙蝠衍生的狂犬病病毒。
Objective To examine the susceptibility of the grey-headed flying fox (Pteropus poliocephalus) to Australian bat lyssavirus (ABL), and to provide preliminary observations on the pathogenesis of the disease in flying foxes.Procedure Ten flying foxes were inoculated intramuscularly with ABL, and four with a bat-associated rabies virus. Inoculated animals were observed daily, and clinical samples collected every 9 to 14 days. Animals with abnormal clinical signs were euthanased, and samples collected for histological, serological, virological and immunohistological examinations. At 3 months post inoculation (PI), all survivors were euthanased, and each submitted to a similar examination.Results Three ABL-inoculated flying foxes, and two rabies-inoculated animals developed abnormal clinical signs between 15 and 24 days P1. All three ABL-inoculated animals had histological lesions consistent with a lyssavirus infection, and lyssaviral antigen was identified in the central nervous system (CNS) of each. Virus was isolated from the brain of two affected animals. Of the rabies-inoculated flying foxes, both had histological lesions and viral antigen in the CNS. Virus was recovered from the brain of only one. None of the five affected flying foxes developed anti-lyssavirus antibodies, but, by 3 months P1, five of the seven ABL-inoculated survivors, and one of the two rabies virus-inoculated survivors, had seroconverted. The dynamics of the immune responses were quite variable.Conclusions The response of flying foxes to ABL, administered by a peripheral route of inoculation, was similar to that of bats inoculated peripherally with bat-derived rabies viruses.