Cell dynamics in the pulpal healing process following cavity preparation in rat molars
Cell dynamics in the pulpal healing process following cavity preparation in rat molars
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DOI：
10.1007/s00418-008-0438-3
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发表时间：
2008-10-01
影响因子：
2.3
通讯作者：
Ohshima, Hayato
中科院分区：
文献类型：
--
作者：
Harada, Masahiro;Kenmotsu, Shin-ichi;Ohshima, Hayato
成牙本质细胞系细胞在完成细胞分裂后获得热休克蛋白（HSP）-25-免疫反应性（IR），这表明该蛋白在牙齿发育过程中充当细胞增殖和分化之间的开关。然而，很少有可用的数据有关的细胞增殖和分化之间的关系洞准备。本研究旨在通过HSP-25免疫细胞化学和5-溴-2 '-脱氧尿嘧啶核苷（BrdU）标记的细胞增殖实验，探讨HSP-25在成牙本质细胞系细胞中的表达及其在成牙本质细胞系细胞中的增殖活性。在未处理的对照牙中，在成牙本质细胞和一些亚成牙本质细胞间充质细胞中发现强烈的HSP-25-IR。洞准备造成破坏的成牙本质细胞和HSP-25-IR的消失是明显的在受影响的网站，虽然一些细胞保留HSP-25-IR，随后他们中的大多数人消失，从牙髓牙本质边界术后第1天。相反，一些HSP-25-IR弱的亚成牙本质细胞间充质细胞开始取代变性细胞，尽管在牙髓中没有增殖活性。有趣的是，牙髓中的增殖细胞在第2天显著增加，此时新分化的细胞已经沿着牙髓-牙本质边界排列，并且在牙髓组织的广泛范围内继续其增殖活性，直到第5天。这些结果表明，在subodontoblastic层配备的祖细胞首先迁移和分化成新的成牙本质细胞样细胞，以弥补成牙本质细胞层的损失，随后牙髓的重组是通过活跃的间充质细胞的增殖发生在广泛的牙髓组织完成。
Odontoblast-lineage cells acquire heat-shock protein (HSP)-25-immunoreactivity (IR) after they complete their cell division, suggesting that this protein acts as a switch between cell proliferation and differentiation during tooth development. However, there are few available data concerning the relationship between cell proliferation and differentiation following cavity preparation. The present study aims to clarify the expression of HSP-25 in the odontoblast-lineage cells with their proliferative activity after cavity preparation by immunocytochemistry for HSP-25 and cell proliferation assay using 5-bromo-2'-deoxyuridine (BrdU) labeling. In untreated control teeth, intense HSP-25-IR was found in odontoblasts and some subodontoblastic mesenchymal cells. Cavity preparation caused the destruction of odontoblasts and the disappearance of HSP-25-IR was conspicuous at the affected site, although some cells retained HSP-25-IR and subsequently most of them disappeared from the pulp-dentin border by postoperative day 1. Contrary, some subodontoblastic mesenchymal cells with weak HSP-25-IR began to take the place of degenerated cells, although no proliferative activity was recognizable in the dental pulp. Interestingly, proliferative cells in the dental pulp significantly increased in number on day 2 when the newly differentiating cells already arranged along the pulp-dentin border, and continued their proliferative activity in the wide range of the pulp tissue until day 5. These findings indicate that progenitor cells equipped in the subodontoblastic layer firstly migrate and differentiate into new odontoblast-like cells to compensate for the loss of the odontoblast layer, and subsequently the reorganization of dental pulp was completed by active proliferation of the mesenchymal cells occurring in a wide range of pulp tissue.