The biparental transmission of the mitochondrial genome in Chlamydomonas reinhardtii visualized in living cells
The biparental transmission of the mitochondrial genome in Chlamydomonas reinhardtii visualized in living cells
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DOI：
10.1016/s0171-9335(98)80080-0
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发表时间：
1998-10-01
影响因子：
6.6
通讯作者：
Kuroiwa, T
中科院分区：
文献类型：
--
作者：
Nishimura, Y;Higashiyama, T;Kuroiwa, T
在同配子绿藻衣藻中，叶绿体基因组是从mt(+)亲本传递的，而线粒体基因被认为是从mt(-)亲本遗传的。用DAPI(4，6-二氨基-2-苯基吲哚)染色可见叶绿体类核，并观察到幼合子对mt类叶绿体类核的优先消化。然而，线粒体类核从未被可视化，它们的行为只是从遗传和生化研究中推断出来的。我们发现，使用荧光染料SYBR GreenI，线粒体和叶绿体基因组可以在活细胞中同时可视化。通过在体内显示线粒体和叶绿体基因组的能力，可以直接观察年轻受精卵中叶绿体和线粒体类核的数量、分布和行为。利用这种方法，揭示了线粒体基因组的双亲传递。
In the isogamous green alga Chlamydomonas reinhardtii, the chloroplast genome is transmitted from the mt(+) parent, while the mitochondrial genes are believed to be inherited from the mt(-) parent. Chloroplast nucleoids have been visualized by DAPI (4,6-diamidino-2-phenylindole) staining, and the preferential digestion of the mt chloroplast nucleoids has been observed in young zygotes. However, the mitochondrial nucleoids have never been visualized, and their behavior is only deduced from genetic and biochemical studies.We discovered that the mitochondrial and chloroplast genomes can be visualized simultaneously in living cells, using the fluorescent dye SYBR GreenI. The ability to visualize the mitochondrial and chloroplast genome in vivo permits the direct observation of the number, distribution and behavior of the chloroplast and mitochondrial nucleoids in young zygotes. Using this method, the biparental transmission of the mitochondrial genome was revealed.