MEASUREMENT OF POOLS OF PROTEIN, CARBOHYDRATE AND LIPID IN DOMESTIC WASTE-WATER
MEASUREMENT OF POOLS OF PROTEIN, CARBOHYDRATE AND LIPID IN DOMESTIC WASTE-WATER
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DOI：
10.1016/0043-1354(94)90261-5
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发表时间：
1994-02-01
期刊：
影响因子：
12.8
通讯作者：
NIELSEN, PH
中科院分区：
文献类型：
--
作者：
RAUNKJAER, K;HVITVEDJACOBSEN, T;NIELSEN, PH
需要对生活污水中的有机物进行比BOD、COD和TOC更详细的表征，以增加对污水系统中的变化和作为废水处理过程输入的废水质量的了解。本文介绍和讨论了对生活污水中碳水化合物、蛋白质和脂肪池进行优化分析的各种选定方法的结果。最适合蛋白质测定的方法是Lowry等人的方法。(1951)，对于碳水化合物，它是由Dreywood(1946)最初引入的蒽酮法，而对于脂类，它是从标准方法(APHA ct al，1985)改进而来的红外法。改进后的方法精密度高，废水中的化合物似乎不干扰测定。然而，这些方法的结果受到标准选择的影响很小。利用不同特征的集水区废水的表征方法进行了实例研究。总蛋白质、碳水化合物和脂肪占总COD的比例平均为28%、18%和31%，标准偏差分别为4%、6%和10%。溶解碳水化合物的比例变化很大--溶解COD的平均值为10%，标准差为7%，这可能是由于停留时间的差异影响了下水道的降解。
A more detailed characterization of organic matter in domestic wastewater than given by the bulk parameters BOD, COD and TOC is needed to increase the knowledge about transformations in sewer systems and the quality of wastewater as input to the wastewater treatment processes. In this paper results of various selected methods optimized for analysing pools of carbohydrate, protein and lipid in domestic wastewater were described and discussed. The most suitable method for measurement of protein was the method of Lowry et al. (1951), for carbohydrate it was the anthrone method originally introduced by Dreywood (1946) and for lipid it was an infrared method modified from Standard Methods (APHA ct al., 1985). The modified methods showed high precision and compounds in the wastewater did not seem to interfere. The results of the methods were, however, slightly influenced by the choice of standard.A case study was performed using the methods for characterization of wastewater from catchment areas of different characteristics. The fractions of total protein, carbohydrate and lipid of total COD were, on average, 28, 18 and 31% with standard deviations of 4, 6 and 10%, respectively. The dissolved carbohydrate fraction varied considerably-10% as an average of dissolved COD, and 7% as standard deviation, presumably due to degradation in the sewers influenced by differences in residence times.