Multimodal nonlinear spectral microscopy based on a femtosecond Cr:forsterite laser
Multimodal nonlinear spectral microscopy based on a femtosecond Cr:forsterite laser
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DOI：
10.1364/ol.26.001909
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发表时间：
2001-12-01
期刊：
影响因子：
3.6
通讯作者：
Lin, BL
中科院分区：
文献类型：
--
作者：
Chu, SW;Chen, IH;Lin, BL
我们展示了一种基于 1230 nm 飞秒 Cr:镁橄榄石激光器的新型多模态非线性光谱显微镜。通过采集可见光和近近红外区域的整个非线性光谱，这项新技术可以组合不同的成像模式，包括二次谐波生成、三次谐波生成和多光子荧光。结合位于红外透明窗口中的选定激发波长，这种显微技术可以提供高穿透深度并减少损伤，是研究活细胞的理想选择。 (C) 2001 年美国光学学会。
We demonstrate a novel multimodal nonlinear spectral microscopy based on a femtosecond Cr:forsterite laser at 1230 nm. By acquiring the whole nonlinear spectrum in the visible and near-NIR region, this novel technique allows a combination of different imaging modalities, including second-harmonic generation, third-harmonic generation, and multiple-photon fluorescence. Combined with the selected excitation wavelength, which is located in the IR transparency window, this microscopic technique can provide high penetration depth with reduced damage and is ideal for studying living cells. (C) 2001 Optical Society of America.