Fine‐scale spatial patterns in microbial community composition in an acid mine drainage
Fine‐scale spatial patterns in microbial community composition in an acid mine drainage
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DOI：
10.1093/femsec/fix124
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发表时间：
2017-10
影响因子：
4.2
通讯作者：
Jie-Liang Liang-Jie-Liang-Liang-37388991;Xiao-Jing Li;Hao-yue Shu;Pandeng Wang;Jialiang Kuang;Jun Liu;Miao-Miao Zhang-Miao;W. Shu;Li-Nan Huang
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文献类型：
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作者：
Jie-Liang Liang-Jie-Liang-Liang-37388991;Xiao-Jing Li;Hao-yue Shu;Pandeng Wang;Jialiang Kuang;Jun Liu;Miao-Miao Zhang-Miao;W. Shu;Li-Nan Huang
摘要微生物群落组成对水生生态系统的功能至关重要，已在不同的环境和不同的空间尺度上进行了研究。然而，记录在案的模式通常基于空间/地理上分离的地点或地点的样本。在此，我们将采样量定义为空间尺度，并用Illumina 16S rRNA测序方法检测了酸性矿山废水中1毫升至10 L尺度上的微生物群落组成。-多样性分析表明，所有样本按照空间尺度非常紧密地分组，每两个尺度之间的差异显著。值得注意的是，每一类群内的平均多样性与空间尺度呈负相关，表明微生物分布呈斑块状。多样性的划分进一步表明，一些微生物类群的相对丰度在空间尺度上变化很大。系统发育分析表明，微生物谱系并不是随机分布的，而是在较小的空间尺度上表现出更多的系统发育聚集性。因此，我们记录了连续水生环境中微生物群落组成的精细空间格局，这可能对未来研究中对水生系统进行充分采样具有实际意义。
ABSTRACT Microbial community composition is essential for aquatic ecosystem functions and has been explored across diverse environments and various spatial scales. However, documented patterns are often based on samples from spatially/geographically separated locations or sites. Here, we define sampling volume as spatial scale and examine (by Illumina 16S rRNA sequencing) microbial community composition over a scale of 1 mL to 10 L in an acid mine drainage. ‐Diversity analysis revealed that all samples grouped very tightly according to spatial scales and variations between every two scales were significant. Notably, mean ‐diversity within each group was negatively correlated with spatial scales, indicating patchy microbial distribution. Partition of ‐diversity further revealed that it was the relative abundances of some microbial taxa that largely changed among spatial scales. Phylogenetic analysis showed that microbial lineages were not randomly distributed, but displayed a tendency of more phylogenetically clustering at smaller spatial scales. Thus, we documented fine‐scale spatial patterns in microbial community composition within a continuous aquatic environment, which may have practical implications for adequate sampling of aquatic systems in future studies.