Chemical investigation of metabolites produced by an endophytic Aspergillus sp isolated from Limonia acidissima
Chemical investigation of metabolites produced by an endophytic Aspergillus sp isolated from Limonia acidissima
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DOI：
10.1080/14786419.2015.1025230
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发表时间：
2015-07-18
影响因子：
2.2
通讯作者：
Fujimoto, Yoshinori
中科院分区：
文献类型：
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作者：
Siriwardane, A. M. D. A.;Kumar, N. Savitri;Fujimoto, Yoshinori
植物内生真菌被认为是产生具有重要生物活性的次生代谢产物的良好来源。在我们对斯里兰卡植物群中环境友好的生物活性化合物的研究的继续中，我们研究了从流行的食用水果Limonia acidissima L.的种子中分离的内生真菌曲霉属（Aspergillus sp.）属于芸香科。曲霉属的纯培养物在马铃薯葡萄糖肉汤培养基上生长。发酵4周后，用有机溶剂提取真菌培养基。经硅胶柱层析、Sephadex LH-20凝胶柱层析和反相高效液相色谱分离，得到黄烷哌酮（1）、红镰孢菌素B（2）、金松酮A（3）、芳塞酮D（4）和金松酮B（5）。化合物1 - 4在卤虫毒性试验中表现出中等活性。这是首次报道化合物4和5的~（13）C NMR数据。
Endophytic fungi are considered as a good source to produce important secondary metabolites with interesting bioactivities. In a continuation of our studies towards the search for environmentally friendly bioactive compounds from Sri Lankan flora, we investigated the secondary metabolites produced by the endophytic fungi Aspergillus sp. isolated from the seeds of the popular edible fruit Limonia acidissima L. of the family Rutaceae. The pure culture of the Aspergillus sp. was grown on potato dextrose broth media. After 4 weeks fermentation, fungal media were extracted with organic solvents. Chromatographic separation of the fungal extracts over silica gel, Sephadex LH-20 and RP-HPLC furnished flavasperone (1), rubrofusarin B (2), aurasperone A (3), fonsecinone D (4) and aurasperone B (5). Compounds 1-4 showed moderate activities in brine shrimp toxicity assay. This is the first report of the C-13 NMR data of compounds 4 and 5.