Pea Xyloglucan and Cellulose IV. Assembly of β-Glucans by Pea Protoplasts
Pea Xyloglucan and Cellulose IV. Assembly of β-Glucans by Pea Protoplasts
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豌豆木葡聚糖和纤维素 IV。通过豌豆原生质体组装 β-葡聚糖。
DOI：
10.1104/pp.82.1.301
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发表时间：
1986
期刊：
影响因子：
7.4
通讯作者：
G. Maclachlan
中科院分区：
文献类型：
--
作者：
Takahisa Hayashi;D. Polonenko;A. Camirand;G. Maclachlan
木葡聚糖的合成和组装过程中的早期阶段的壁再生的原生质体分离黄化豌豆的生长区域进行了检查。在培养的早期阶段，荧光显微镜显示，原生质体表面结合Calcofluor和铵盐的8-苯胺基-1-萘磺酸，并在时间，它也结合荧光岩藻糖结合凝集素。化学分析表明，原生质体形成的主要多糖为1，3-β-葡聚糖，其次为木葡聚糖和纤维素。纤维素和木葡聚糖之间的结合不如完整豌豆植物组织中的结合那么强，即弱碱可以从原生质体中溶解大部分木葡聚糖。然而，添加外源豌豆木葡聚糖到培养基中刺激新的多糖沉积到原生质体壁中，并增强新形成的木葡聚糖与纤维素的紧密结合。
The synthesis and assembly of xyloglucan were examined during early stages of wall regeneration by protoplasts isolated from growing regions of etiolated peas. During early stages of cultivation, fluorescence microscopy showed that the protoplast surface bound Calcofluor and ammonium salt of 8-anilino-1-naphthalene sulfonic acid and, in time, it also bound fluorescent fucose-binding lectin. Based on chemical analysis, 1,3-β-glucan was the main polysaccharide formed by protoplasts and xyloglucan and cellulose were minor wall components. Binding between cellulose and xyloglucan was not as strong as that in tissues of intact pea plants, i.e. mild alkali could dissolve most xyloglucan from the protoplast. However, the addition of exogenous pea xyloglucan into the culture medium stimulated the deposition of new polysaccharides into the protoplast wall and enhanced the close association of newly formed xyloglucan with cellulose.