Bivalent Formation 1, a plant-conserved gene, encodes an OmpH/coiled-coil motif-containing protein required for meiotic recombination in rice.
Bivalent Formation 1, a plant-conserved gene, encodes an OmpH/coiled-coil motif-containing protein required for meiotic recombination in rice.
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二价形成 1 是一种植物保守基因，编码水稻减数分裂重组所需的含有 OmpH/卷曲螺旋基序的蛋白质。
DOI：
10.1093/jxb/erx077
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发表时间：
2017-04-01
影响因子：
6.9
通讯作者：
Liu YG
中科院分区：
文献类型：
--
作者：
Zhou L;Han J;Chen Y;Wang Y;Liu YG
OsBVF 1是单子叶水稻中一个新的减数分裂重组所必需的基因。减数分裂是真核生物有性生殖和植物育性的基本过程。与拟南芥中鉴定的80多个减数分裂基因相比，水稻中鉴定的减数分裂基因只有约30个。许多参与减数分裂进程调控的基因仍有待确定。本研究鉴定了一个水稻不育突变体，并通过图位克隆的方法克隆了一个新的减数分裂基因OsBVF 1（Bivalent Formation 1）。利用分子遗传学和细胞学方法研究OsBVF 1在减数分裂中的功能。利用系统发育分析方法研究OsBVF 1及其同源基因在植物中的进化。我们发现bvf 1雄母细胞减数分裂双链断裂的形成缺陷，从而导致终变期不能形成二价体和后期I染色体不均等分离。致病基因OsBVF 1编码一种独特的OmpH/卷曲螺旋基序蛋白，其同源物在植物界高度保守，在大多数植物物种中似乎是单拷贝基因。我们的研究表明OsBVF 1是一个新的植物保守因子，参与水稻减数分裂重组，为理解减数分裂进程调控提供了新的见解。
OsBVF1 is a novel gene that is required for meiotic recombination in the monocot rice. Meiosis is essential for eukaryotic sexual reproduction and plant fertility. In comparison with over 80 meiotic genes identified in Arabidopsis, there are only ~30 meiotic genes characterized in rice (Oryza sativa L.). Many genes involved in the regulation of meiotic progression remain to be determined. In this study, we identified a sterile rice mutant and cloned a new meiotic gene, OsBVF1 (Bivalent Formation 1) by map-based cloning. Molecular genetics and cytological approaches were carried out to address the function of OsBVF1 in meiosis. Phylogenetic analyses were used to study the evolution of OsBVF1 and its homologs in plant species. Here we showed that the bvf1 male meiocytes were defective in formation of meiotic double strand break, thereby resulting in a failure of bivalent formation in diakinesis and unequal chromosome segregation in anaphase I. The causal gene, OsBVF1, encodes a unique OmpH/coiled-coil motif-containing protein and its homologs are highly conserved in the plant kingdom and seem to be a single-copy gene in the majority of plant species. Our study demonstrates that OsBVF1 is a novel plant-conserved factor involved in meiotic recombination in rice, providing a new insight into understanding of meiotic progression regulation.