Long-term cigarette smoke exposure increases uncoupling protein expression but reduces energy intake
Long-term cigarette smoke exposure increases uncoupling protein expression but reduces energy intake
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DOI：
10.1016/j.brainres.2008.06.067
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发表时间：
2008-09-04
期刊：
影响因子：
2.9
通讯作者：
Morris, Margaret J.
中科院分区：
文献类型：
--
作者：
Chen, Hui;Hansen, Michelle J.;Morris, Margaret J.
烟草的食欲抑制作用是吸烟行为的主要驱动力;然而，很少有研究涉及慢性香烟烟雾暴露（SE）对食欲，体重和代谢标志物的影响。我们比较了SE与同等食物限制（成对喂养，PF）对雄性Balb/C小鼠体重、肥胖、细胞因子以及解偶联蛋白（UCP）和脑神经肽Y（NPY）水平的影响。SE迅速引起厌食，12周后，SE和PF组比对照组动物轻（分别为23.9 +/- 0.2，25.5 +/- 0.5，26.8 +/- 0.4 g，P < 0.05）。白色脂肪（WAT）质量减少SE和PF。血浆瘦素和胰岛素减少SE小鼠;胰岛素进一步减少PF。棕色脂肪UCP 1和3 mRNA增加SE动物相对于PF动物，可能促进产热。SE使炎性细胞因子TNF α的WAT mRNA表达加倍，而PF和SE均降低IL-6。SE使下丘脑NPY含量增加（89.3 +/- 2.8对75.9 +/- 2.4 ng对照，P < 0.05），PF使下丘脑NPY含量增加更多（100.7 +/- 3.4 ng，与两组相比P < 0.05），表明由于脂肪来源的瘦素减少而导致抑制解除。与同等的食物限制相比，香烟烟雾暴露降低了体重和总下丘脑NPY，并增加了产热和炎症标志物。下丘脑NPY的抑制和UCPs的增加可能是导致SE动物自发性食欲减退和额外体重减轻的原因。这些发现有助于我们理解吸烟相关肺部疾病的体重减轻，这表明其影响比单纯厌食症更大。皇冠版权所有（C）2008年出版的爱思唯尔B. V.保留所有权利。
The appetite suppressing effect of tobacco is a major driver of smoking behaviour; however few studies have addressed the effects of chronic cigarette smoke exposure (SE) on appetite, body weight and metabolic markers. We compared the effects of SE to equivalent food restriction (pair-fed, PF), against sham-exposure, on body weight, adiposity, cytokines, and levels of uncoupling proteins (UCP) and brain neuropeptide Y (NPY) in male Balb/C mice. SE rapidly induced anorexia, and after 12 weeks, SE and PF groups were lighter than control animals (23.9 +/- 0.2, 25.5 +/- 0.5, 26.8 +/- 0.4 g respectively, P < 0.05). White fat (WAT) masses were reduced by both SE and PF. Plasma leptin and insulin were reduced in SE mice; insulin was further reduced by PF. Brown fat UCP1 and 3 mRNA were increased in SE animals relative to PF animals, possibly promoting thermogenesis. WAT mRNA expression of the inflammatory cytokine, TNF alpha was doubled by SE, while IL-6 was reduced by both PF and SE. Hypothalamic NPY content was increased by SE (89.3 +/- 2.8 vs. 75.9 +/- 2.4 ng control, P < 0.05), and more by PF (100.7 +/- 3.4 ng, P < 0.05 compared to both groups), suggesting disinhibition due to reduced adipose derived leptin. In contrast to equivalent food restriction, cigarette smoke exposure reduced body weight and total hypothalamic NPY, and increased thermogenesis and markers of inflammation. The suppressed hypothalamic NPY and increased UCPs may contribute to the spontaneous hypophagia and extra weight loss in SE animals. These findings contribute to our understanding of weight loss in smoking-related lung disease, suggesting a greater impact than that due to anorexia alone. Crown Copyright (C) 2008 Published by Elsevier B.V. All rights reserved.