Mal3 Masks Catastrophe Events in Schizosaccharomyces pombe Microtubules by Inhibiting Shrinkage and Promoting Rescue
Mal3 Masks Catastrophe Events in Schizosaccharomyces pombe Microtubules by Inhibiting Shrinkage and Promoting Rescue
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DOI：
10.1074/jbc.c109.052159
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发表时间：
2009-10-23
影响因子：
4.8
通讯作者：
Cross, Robert A.
中科院分区：
文献类型：
--
作者：
Katsuki, Miho;Drummond, Douglas R.;Cross, Robert A.
粟酒裂殖酵母Mal3是EB蛋白家族的成员，其被认为是调节细胞中微管动力学的尖端跟踪网络中的核心元件。Mal3本身如何影响微管动力学尚不清楚。我们检测了全长重组Mal3对纯化的S. pombe微管蛋白，使用暗场视频显微镜，以避免荧光标记和数据平均技术，以提高时空分辨率。我们发现灾难的发生是随机的，就像一个快速的（
Schizosaccharomyces pombe Mal3 is a member of the EB family of proteins, which are proposed to be core elements in a tip-tracking network that regulates microtubule dynamics in cells. How Mal3 itself influences microtubule dynamics is unclear. We tested the effects of full-length recombinant Mal3 on dynamic microtubules assembled in vitro from purified S. pombe tubulin, using dark field video microscopy to avoid fluorescent tagging and data-averaging techniques to improve spatiotemporal resolution. We find that catastrophe occurs stochastically as a fast (