Association between the adiponectin promoter rs266729 gene variant and oxidative stress in patients with diabetes mellitus
Association between the adiponectin promoter rs266729 gene variant and oxidative stress in patients with diabetes mellitus
复制标题
DOI：
10.1093/eurheartj/ehp090
复制
发表时间：
2009-05-01
影响因子：
39.3
通讯作者：
Stephens, Jeffrey W.
中科院分区：
文献类型：
--
作者：
Prior, Sarah L.;Gable, David R.;Stephens, Jeffrey W.
低水平的脂联素与2型糖尿病和冠心病（CHD）有关。最近的证据还表明，低水平的脂联素与氧化应激增加有关。我们的目的是研究rs266729启动子基因变异（-11377C > G）与糖尿病患者血浆氧化应激标志物之间的关系。767例高加索糖尿病患者成功进行基因分型（CC/CG/GG）。分析基因型数据与血浆总抗氧化状态（TAOS）和氧化低密度脂蛋白（Ox-LDL）的关系。在206个样本中进行了血浆脂联素测量。基因型与血浆TAOS有显著相关性（CC: 42.1 +/- 13.4% vs. CG: 42.0 +/- 12.0% vs. GG: 47.9 +/- 12.0%, P = 0.02; CC/CG vs. GG, P = 0.006）。在Ox-LDL方面，CC受试者的血浆Ox-LDL比CG/GG高8% [CC vs CG vs GG: 48.5 (36.3-60.2) U/L vs. 44.8 (35.6-54.1) U/L vs. 44.9 (41.2-49.1) U/L, CC vs CG/GG P = 0.03]。血浆脂联素水平，GG组最高[CC vs. CG vs. GG: 8.18 (5.69 ~ 15.38) mu g/mL vs. 7.12 (5.34 ~ 12.97) mu g/mL vs. 11.84 (6.98 ~ 25.25) mu g/mL, P = 0.09；CC/CG与GG， P = 0.05]。本研究显示了脂联素基因启动子变异与血浆氧化应激标志物之间的关联。与先前的研究一致，这项工作支持脂联素的抗氧化作用，这可能解释了它的心脏保护作用。需要进一步的前瞻性研究来探讨该基因变异在糖尿病中与冠心病风险和氧化应激的关系。
Low levels of adiponectin are associated with type 2 diabetes and coronary heart disease (CHD). Recent evidence also suggests that low levels of adiponectin are associated with increased oxidative stress. Our aim was to examine the association between the rs266729 promoter gene variant (-11377C > G) and plasma markers of oxidative stress in diabetes subjects.Seven hundred and sixty-seven Caucasian subjects with diabetes were successfully genotyped (CC/CG/GG). Genotype data were analysed in relation to plasma total antioxidant status (TAOS) and Oxidized-LDL (Ox-LDL). Plasma adiponectin measurements were available in 206 samples. There was a significant association between genotype and plasma TAOS (CC: 42.1 +/- 13.4% vs. CG: 42.0 +/- 12.0% vs. GG: 47.9 +/- 12.0%, P = 0.02; for CC/CG vs. GG, P = 0.006). With respect to Ox-LDL, CC subjects had 8% higher plasma Ox-LDL compared with CG/GG [CC vs. CG vs. GG: 48.5 (36.3-60.2) U/L vs. 44.8 (35.6-54.1) U/L vs. 44.9 (41.2-49.1) U/L, for CC vs. CG/GG P = 0.03]. For plasma adiponectin, GG subjects had the highest levels [CC vs. CG vs. GG: 8.18 (5.69-15.38) mu g/mL vs. 7.12 (5.34-12.97) mu g/mL vs. 11.84 (6.98-25.25) mu g/mL, P = 0.09; for CC/CG vs. GG, P = 0.05].This study shows an association between a promoter variant in the adiponectin gene and plasma markers of oxidative stress. In line with previous studies, this work supports an antioxidant role for adiponectin which may explain its cardioprotective effect. Further prospective study is necessary to explore the effect of this gene variant in diabetes in relation to CHD risk and oxidative stress.