DNA methylation by CcrM activates the transcription of two genes required for the division of Caulobacter crescentus.
DNA methylation by CcrM activates the transcription of two genes required for the division of Caulobacter crescentus.
复制标题
DOI：
10.1111/mmi.12180
复制
发表时间：
2013-04
影响因子：
3.6
通讯作者：
Collier J
中科院分区：
文献类型：
--
作者：
Gonzalez D;Collier J
DNA甲基化通过在DNA序列上叠加二级信息来调节许多过程，包括基因表达。保守的CCRM酶负责甲基化GANTC序列中的腺嘌呤，对几种甲型变形杆菌的生存是必不可少的。在本研究中，我们发现缺乏CCRM酶的新月桂杆菌细胞积累了低水平的两个保守的FtsZ和MipZ蛋白，导致细胞分裂严重缺陷。这一缺陷可以通过可诱导启动子的FtsZ基因的表达或通过促进FtsZ积累的自发抑制突变来弥补。我们发现CCRM促进FtsZ和mipZ基因的转录，FtsZ和mipZ启动子区域包含一个保守的CGACTC基序，该基序对它们的活性和CCRM对它们的调节至关重要。此外，我们的结果表明，当CGACTC基序未甲基化时，FtsZ启动子的活性最低，半甲基化时活性居中，完全甲基化时活性最高。DNA甲基化对FtsZ表达的调节可能解释了为什么CCRM在阿尔法变形杆菌的一个子集中是必不可少的。
DNA methylation regulates many processes, including gene expression, by superimposing secondary information on DNA sequences. The conserved CcrM enzyme, which methylates adenines in GANTC sequences, is essential to the viability of several Alphaproteobacteria. In this study, we find that Caulobacter crescentus cells lacking the CcrM enzyme accumulate low levels of the two conserved FtsZ and MipZ proteins, leading to a severe defect in cell division. This defect can be compensated by the expression of the ftsZ gene from an inducible promoter or by spontaneous suppressor mutations that promote FtsZ accumulation. We show that CcrM promotes the transcription of the ftsZ and mipZ genes and that the ftsZ and mipZ promoter regions contain a conserved CGACTC motif that is critical to their activities and to their regulation by CcrM. In addition, our results suggest that the ftsZ promoter has the lowest activity when the CGACTC motif is non-methylated, an intermediate activity when it is hemi-methylated and the highest activity when it is fully methylated. The regulation of ftsZ expression by DNA methylation may explain why CcrM is essential in a subset of Alphaproteobacteria.
登录
查看更多内容
影响因子：
4.5
作者：
Brunet YR;Bernard CS;Gavioli M;Lloubès R;Cascales E
通讯作者：
Cascales E
DOI：
10.1073/pnas.96.19.10661
发表时间：
1999-09-14
影响因子：
11.1
作者：
Jensen, RB;Shapiro, L
通讯作者：
Shapiro, L
影响因子：
3.2
作者：
Marczynski, GT
通讯作者：
Marczynski, GT
影响因子：
3.2
作者：
Kahng, LS;Shapiro, L
通讯作者：
Shapiro, L
影响因子：
4.5
作者：
Demarre G;Chattoraj DK
通讯作者：
Chattoraj DK