Oral administration of lactic acid bacteria isolated from traditional South Asian fermented milk 'dahi' inhibits the development of atopic dermatitis in NC/Nga mice.
Oral administration of lactic acid bacteria isolated from traditional South Asian fermented milk 'dahi' inhibits the development of atopic dermatitis in NC/Nga mice.
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口服从传统南亚发酵奶“dahi”中分离出的乳酸菌可抑制 NC/Nga 小鼠特应性皮炎的发展。
DOI：
10.3177/jnsv.55.271
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发表时间：
2009
影响因子：
1.6
通讯作者：
T. Miyamoto
中科院分区：
文献类型：
--
作者：
Toyoaki Watanabe;K. Hamada;A. Tategaki;H. Kishida;Hozumi Tanaka;M. Kitano;T. Miyamoto
采用NC/Nga AD模型小鼠，研究了从传统南亚发酵乳“大奶”中分离的乳酸菌（LAB）对特应性皮炎（AD）发展的抑制作用。在初步评价中，我们利用卵清蛋白（OVA）诱导的1型过敏模型小鼠，证实了乳酸菌对血清总IgE的影响。在ova致敏小鼠脾细胞中，对41株从大喜中分离出来的细菌进行了诱导白细胞介素(IL)-12产生和抑制IL-4产生的能力评估。在所检测的41株菌株中，德尔布鲁氏乳杆菌亚种。lactis R-037对IL-12的诱导作用最强，提示其是一种有效的Th1诱导剂。经热处理的R-037可显著抑制ova诱导的1型过敏模型小鼠血清总IgE的升高。在NC/Nga AD模型小鼠中，口服热处理R-037可减轻炎性耳廓厚度，减轻AD临床评分，但对血清总IgE水平的影响尚不清楚。组织病理学结果显示炎症有改善的趋势。角化过度在皮炎和皮肤损伤中表现出明显的改善。这些结果提示口服R-037可能减轻AD。
We investigated the suppressive effect of lactic acid bacteria (LAB) isolated from traditional South Asian fermented milk 'dahi' on the development of atopic dermatitis (AD) using NC/Nga AD model mice. In the initial evaluation, we confirmed the effect of LAB on serum total IgE using ovalbumin (OVA)-induced type 1 allergy model mice. Forty-one bacterial strains isolated from dahi were evaluated for their ability to induce interleukin (IL)-12 production and suppress IL-4 production in splenocytes obtained from OVA-sensitized mice. Of the 41 strains tested, Lactobacillus delbrueckii subsp. lactis R-037 exhibited the greatest IL-12 induction, suggesting that it is a potent Th1 inducer. Oral administration of heat-treated R-037 significantly suppressed the elevation of serum total IgE in OVA-induced type 1 allergy model mice. In NC/Nga AD model mice, oral administration of heat-treated R-037 reduced inflammatory auricular thickness and alleviated the AD clinical score although the effect on serum total IgE level was unclear. Histopathological findings showed a tendency toward improvement of inflammation. Hyperkeratosis in particular showed improvement in dermatitis skin lesions. These results suggest that oral administration of R-037 may alleviate AD.