Carbendazim induces a temporary change in soil bacterial community structure.
Carbendazim induces a temporary change in soil bacterial community structure.
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DOI：
10.1016/s1001-0742(08)62473-0
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发表时间：
2009
影响因子：
6.9
通讯作者：
Xiuguo Wang;M. Song;Chunming Gao;Bin Dong;Qun Zhang;H. Fang;Yunlong Yu
中科院分区：
文献类型：
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作者：
Xiuguo Wang;M. Song;Chunming Gao;Bin Dong;Qun Zhang;H. Fang;Yunlong Yu
采用温度梯度凝胶电泳（TGGE）和PCR扩增16 SrRNA基因部分序列分析，研究了田间条件下施用多菌灵对土壤细菌群落多样性和结构的影响。在连续四次以0.94 kg活性成分（a.i.）/ hm-2，遗传多样性指数（Shannon指数，H′）由对照的1.43下降到处理的1.29。这种有害影响似乎随着多菌灵浓度的增加而增加。多菌灵4.70 kg a.i./ ha降至1.05（P ≤ 0.05）。多菌灵0.94、1.88和4.70 kg a.i./kg重复施用4次后，土壤细菌群落结构也受到影响。HA，如在单个条带的相对强度中所见。而多菌灵处理后360 d，土壤细菌群落恢复到对照水平。结果表明，多菌灵的重复施用会降低土壤微生物多样性，暂时改变土壤细菌群落结构。
The effect of carbendazim applications on the diversity and structure of a soil bacterial community was studied under field conditions using temperature gradient gel electrophoresis (TGGE) and partial sequence analysis of PCR-amplified 16S rRNA gene. After four successive introductions of carbendazim at a level of 0.94 kg active ingredient (a.i.)/ha, the genetic diversity (expressed as Shannon index, H′) decreased from 1.43 in the control to 1.29 in treated soil. This harmful effect seems to increase with the concentration of carbendazim. The value of H′ in the soil treated with carbendazim at 4.70 kg a.i./ha was reduced to 1.05 (P ≤ 0.05). The structure of soil bacterial community was also affected after four repeated applications of carbendazim at levels of 0.94, 1.88 and 4.70 kg a.i./ha, as seen in the relative intensities of the individual band. However, the bacterial community in carbendazim-treated soil recovered to that in the control 360 d after the first treatment. The results indicated that repeated applications of carbendazim could reduce soil microbial diversity and alter the bacterial community structure temporarily.