A preparative suspension culture system permitting quantitation of anchorage-independent growth by direct radiolabeling of cellular DNA.
A preparative suspension culture system permitting quantitation of anchorage-independent growth by direct radiolabeling of cellular DNA.
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一种制备型悬浮培养系统，可通过直接放射性标记细胞 DNA 来定量不依赖贴壁的生长。
DOI：
10.1016/0003-2697(89)90020-1
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发表时间：
1989
影响因子：
2.9
通讯作者：
Drosinos,S
中科院分区：
文献类型：
--
作者：
Assoian,RK;Boardman,LA;Drosinos,S
我们已经开发了一种混合甲基纤维素/琼脂悬浮培养系统，它允许转化间充质细胞的长期集落形成。与传统的琼脂悬浮液相比，我们的系统允许回收细胞和直接进行锚定非依赖性生长的生化分析。在这些制备培养物中容易放射性标记细胞大分子的能力允许通过将[3 H]胸苷掺入新合成的DNA中来定量和客观地分析菌落形成。
We have developed a hybrid methylcellulose/agar suspension culture system which permits long-term colony formation of transformed mesenchymal cells. In contrast to traditional agar suspensions, our system allows for recovery of cells and direct biochemical analysis of anchorage-independent growth. The ability to readily radiolabel cellular macromolecules in these preparative cultures permits a quantitative and objective analysis of colony formation by incorporation of [3H]thymidine into newly synthesized DNA.