Absolute configuration of 7,12-dimethylbenz[a]anthracene-DNA adducts in mouse epidermis.
Absolute configuration of 7,12-dimethylbenz[a]anthracene-DNA adducts in mouse epidermis.
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小鼠表皮中 7,12-二甲基苯并[a]蒽-DNA 加合物的绝对构型。
DOI：
10.1016/0304-3835(91)90163-c
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发表时间：
1991
期刊：
影响因子：
9.7
通讯作者：
Dipple,A
中科院分区：
文献类型：
--
作者：
Vericat,JA;Cheng,SC;Dipple,A
32P-Postlabeling用于监测反式3,4-二氢二醇各对映体在小鼠表皮中dba - dna加合物的形成。结果表明，(4R,3R)-二氢二醇转化为抗(4R,3S)-二氢二醇(2S,1R)-环氧化物，与表皮DNA中的脱氧鸟苷和脱氧腺苷残基反应，生成DMBA自身与表皮DNA结合时形成的两种主要加合物。第三种主要的dba衍生的脱氧腺苷加合物是由(4S,3R)-二氢二醇通过syn (4S,3R)-二氢二醇(2S,1R)-环氧化物的中间体产生的。
32P-Postlabeling was used to monitor the formation of DMBA-DNA adducts in mouse epidermis from each enantiomer of the trans 3,4-dihydrodiol. It was shown that the (4R,3R)-dihydrodiol is converted to the anti (4R,3S)-dihydrodiol (2S,1R)-epoxide which reacts with deoxyguanosine and deoxyadenosine residues in epidermal DNA to yield two of the major adducts formed when DMBA itself binds to epidermal DNA. The third major DMBA-derived adduct with deoxyadenosine residues was shown to arise from the (4S,3R)-dihydrodiol through the intermediacy of the syn (4S,3R)-dihydrodiol (2S,1R)-epoxide.